Purification and characterization of oxalate oxidase from wheat seedlings

被引：0
|
作者：
Yihong Hu
Zhenfei Guo
机构：
[1] South China Agricultural University,College of Life Science
来源：
Acta Physiologiae Plantarum | 2009年 / 31卷
关键词：
Characterization; Chemical modification; Oxalate oxidase; Protein purification; Wheat;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Oxalate oxidase (OxO, EC 1.2.3.4.) was purified to homogeneity from wheat (Triticum aestivum) seedlings by sequential thermal treatment, ultrafiltration, Sephadex G-100 gel filtration and affinity chromatography with concanavalin A. The enzyme was purified 66.11-fold with a recovery of 21.97%. It showed a subunit molecular mass of 32.6 kDa on SDS-PAGE and a native molecular mass of 170 kDa on Sephadex G-150 filtration, suggesting that it is a pentamer. The wheat OxO had a maximum activity at pH 3.5. Its Km for oxalate was 0.21 mM. Chemical modification revealed that cysteine, lysine and carboxylate residues were essential for OxO activity, whereas arginine, serine, threonine and tryptophane residues were not essential.
引用
收藏
页码：229 / 235
页数：6
相关论文
共 50 条
  • [31] Partial purification and characterization of polyphenol oxidase and peroxidase from chestnut kernel
    Gong, Zhiqing
    Li, Dajing
    Liu, Chunquan
    Cheng, Anwei
    Wang, Wenliang
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2015, 60 (02) : 1095 - 1099
  • [32] Purification and characterization of latent polyphenol oxidase from truffles (Terfezia arenaria)
    Benaceur, Farouk
    Chaibi, Rachid
    Berrabah, Fethi
    Neifar, Aref
    Leboukh, Mourad
    Benaceur, Kenza
    Nouioua, Wafa
    Rezzoug, Asmaa
    Bouazzara, Houria
    Gouzi, Hicham
    Cabana, Hubert
    Gargouri, Ali
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 145 : 885 - 893
  • [33] Heterologous expression of barley and wheat oxalate oxidase in an E-coli trxB gor double mutant
    Cassland, P
    Larsson, S
    Nilvebrant, NO
    Jönsson, LJ
    JOURNAL OF BIOTECHNOLOGY, 2004, 109 (1-2) : 53 - 62
  • [34] Determination of total oxalate contents of a great variety of foods commonly available in Southern China using an oxalate oxidase prepared from wheat bran
    Ruan, Quan-Yuan
    Zheng, Xue-Qin
    Chen, Bai-Ling
    Xiao, Yang
    Peng, Xin-Xiang
    Leung, David W. M.
    Liu, E-E
    JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2013, 32 (01) : 6 - 11
  • [35] Characterization of recombinant barley oxalate oxidase expressed by Pichia pastoris
    Whittaker, MM
    Whittaker, JW
    JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2002, 7 (1-2): : 136 - 145
  • [36] Calcium oxalate degrading thermophilic oxalate oxidase from newly isolated Fusarium oxysporum RBP3
    Kizhakedathil, Moni Philip Jacob
    Bose, Ronit
    Belur, Prasanna D.
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2020, 25
  • [37] Partially purification and characterization of polyphenol oxidase of quince
    Yagar, H
    Sagiroglu, A
    TURKISH JOURNAL OF CHEMISTRY, 2002, 26 (01): : 97 - 103
  • [38] Purification of sucrose synthase from thermotolerant wheat grains and its characterization
    Verma, E.
    Sharma, B.
    Singal, H. R.
    Munjal, R.
    JOURNAL OF ENVIRONMENTAL BIOLOGY, 2018, 39 (04): : 459 - 466
  • [39] PURIFICATION TO HOMOGENEITY AND CHARACTERIZATION OF HOMOSERINE KINASE FROM WHEAT-GERM
    RIESMEIER, J
    KLONUS, AK
    POHLENZ, HD
    PHYTOCHEMISTRY, 1993, 32 (03) : 581 - 583
  • [40] Purification and characterization of ginsenoside-hydrolyzing β-glucosidase from wheat bran
    Zhang, Chunzhi
    Chen, Ming
    Guo, Haichen
    Zu, Guoren
    Chen, Li
    BIOTECHNOLOGY, CHEMICAL AND MATERIALS ENGINEERING II, PTS 1 AND 2, 2013, 641-642 : 906 - +
12345