Stripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markers

被引：17
作者：
Waris, Mohammad Haider [1 ]
Kaur, Jaspal [2 ]
Bala, Ritu [2 ]
Singh, Sandeep [1 ]
Srivastava, Puja [2 ]
Sharma, Achla [2 ]
Singh, Rohtas [2 ]
Kumari, Jyoti [3 ]
机构：
[1] PAU, Dept Plant Pathol, Ludhiana, India
[2] PAU, Dept Plant Breeding & Genet, Ludhiana, India
[3] Natl Bur Plant Genet Resources, New Delhi, India
来源：
SCIENTIFIC REPORTS | 2023年 / 13卷 / 01期
关键词：
F-SP TRITICI; WILD EMMER WHEAT; PUCCINIA-STRIIFORMIS; YELLOW RUST; MONOSOMIC ANALYSIS; YR15; IDENTIFICATION; POPULATION; YR5; EPIDEMIOLOGY;
D O I：
10.1038/s41598-023-36197-y
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Thirteen known Yr gene-associated markers pertaining to genes (Yr5,Yr10,Yr15,Yr24/Yr26) were used to identify the genes in selected wheat germplasm which were found resistant under field conditions at two locations in Punjab, India against stripe rust. In field evaluation, 38 genotypes exhibited highly resistant response, with a final rust severity (FRS) ranging from 0 to TR. Seven genotypes expressed a resistant to moderately resistant response with FRS ranging from 5MR-10S. In race-specific phenotying against most prevalent pathotypes of Pucciniastriiformistritici (46S119,110S119 &238S119) by seedling reaction test (SRT) 14 genotypes (29.2%) were found to be immune (IT = 0), 28 genotypes (58.3%) were resistant (IT = 1), and 3 genotypes (6.3%) were moderately resistant (IT = 2). Yr5 was detected in sixteen lines with the help of two markers Xwmc175 and Xgwm120 linked with Yr5. Yr10 was detected in ten lines with the marker Xpsp3000 and Yr15 was detected in fourteen lines with two linked markers; Xgwm413 and Xgwm273. Likewise, Yr24/26 was detected in 15 lines with two linked markers, namely Xbarc181 and Xbarc187. Based on the race specific phenotyping data and marker data, fourteen lines were found to carry a single gene, 16 showed the presence of two gene combinations, and seven genotypes were found to have a combination of three genes. Frequencies of Yr5, Yr15 and Yr26/Yr24 was high among test wheat germplasm in comparison to Yr10.
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页数：14
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