Multi-lineage Potential Research of Bone Marrow-Derived Stromal Cells (BMSCs) from Cattle

被引：0
作者：
Taofeng Lu
Yina Huang
Hui Wang
Yuehui Ma
Weijun Guan
机构：
[1] Chinese Academy of Agricultural Sciences,Institute of Animal Sciences
[2] Northeast Forestry University,College of Wildlife Resources
来源：
Applied Biochemistry and Biotechnology | 2014年 / 172卷
关键词：
BMSCs; Multi-lineage differentiation; Hepatic cells; Islet-like cells;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Bovine bone marrow-derived mesenchymal stem cells (bBMSCs) were isolated from the bone marrow of a 4–6-month-old fetal bovine and then characterized by immunofluorescence and reverse transcriptase polymerase chain reaction. We found that primary bBMSCs could be expanded for 46 passages; the total culture time in vitro was 125 days. The results of surface antigen detection showed that bBMSCs expressed CD29, CD44, and CD73 but did not express endothelial cells and hematopoietic cells-specific marker CD31, CD34, and CD45. The cells from four passages (passages 3, 9, 15, and 25) were successfully induced to differentiate into osteoblasts, adipocytes, hepatic, and islet-like cells. The results indicate the potential for multi-lineage differentiation of bBMSCs that may represent an ideal candidate for cellular transplantation therapy.
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页码：21 / 35
页数：14
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