Transgenic elite Indica rice varieties, resistant to Xanthomonas oryzae pv. oryzae

被引：0
作者：
Shiping Zhang
Wen-Yuan Song
Lili Chen
Deling Ruan
Nigel Taylor
Pamela Ronald
Roger Beachy
Claude Fauquet
机构：
[1] International Laboratory for Tropical Agricultural Biotechnology (ILTAB/ORSTOM-TSRI),Division of Plant Biology
[2] University of California,BCC 206, The Scripps Research Institute
来源：
Molecular Breeding | 1998年 / 4卷
关键词：
Indica rice; cell suspension; transformation; Xa21; bacterial leaf blight;
D O I：
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中图分类号：
学科分类号：
摘要：
The agronomically important Indica (group 1) rice varieties IR64, IR72, hybrid restorer line Minghui 63, and BG90-2 were co-transformed by microbombardment of embryogenic suspensions with plasmids that contain the Xa21 gene which confers resistance to Xanthomonas oryzae pv. oryzae and the hph gene for resistance to hygromycin B. Six of the 55 transgenic R0 plant lines containing the Xa21 gene displayed high levels of resistance to the pathogen, and no partial resistance was observed. The trait was stably inherited in subsequent generations, and transgenic plants are currently in field tests. The ability to transfer agronomically important genes into elite Indica rice varieties demonstrates the applicability of genetic engineering for the agronomic improvement of rice.
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页码：551 / 558
页数：7
相关论文
共 75 条
[1]  
Christou P(1991)Production of transgenic rice ( Bio/technology 9 957-962
[2]  
Ford TL(1983) L.) plants from agronomically important Plant Mol Biol Rep 1 19-21
[3]  
Kofron M(1993) and Bio/technology 11 1151-1155
[4]  
Dellaporta SL(1968) varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos Exp Cell Res 50 151-158
[5]  
Wood J(1987)A plant DNA minipreparation: Version II Theo Appl Genet 74 21-30
[6]  
Hicks JB(1992)Insect resistant rice generated by introduction of a modified ∂-endotoxin gene of Proc Natl Acad Sci USA 89 9865-9869
[7]  
Fujimoto H(1973)Nutrient requirements of suspension cultures of soybean root cells Plant Dis Rep 57 537-541
[8]  
Itoh K(1990)Isozyme and classification of Asian rice varieties Rice Genet Newsl 17 121-122
[9]  
Yamamoto M(1962)Genetically engineered rice resistant to rice strip virus, an insect-transmitted virus Physiol Plant 15 473-497
[10]  
Kyozuka J(1973)An improved technique for evaluation of resistance of rice varieties to Plant Cell Physiol 14 1113-1121
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