Genomic sequence analysis of Helicoverpa armigera nucleopolyhedrovirus isolated from Australia

被引:8
作者
Zhang H. [1 ]
Yang Q. [1 ,3 ]
Qin Q.-L. [1 ]
Zhu W. [1 ]
Zhang Z.-F. [2 ]
Li Y.-N. [2 ]
Zhang N. [1 ]
Zhang J.-H. [1 ]
机构
[1] State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing
[2] Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing
[3] Henan Jiyuan Baiyun Industry Co., Ltd, Henan
关键词
Nucleotide Sequence Identity; Virus Species; Cotton Bollworm; Nucleocytoplasmic Shuttle; Nucleotide Variability;
D O I
10.1007/s00705-013-1823-3
中图分类号
学科分类号
摘要
The complete genomic sequence of Helicoverpa armigera nucleopolyhedrovirus from Australia, HearNPV-Au, was determined and analyzed. The HearNPV-Au genome was 130,992 bp in size with a G + C content of 39 mol% and contained 134 predicted open reading frames (ORFs) consisting of more than 150 nucleotides. HearNPV-Au shared 94 ORFs with AcMNPV, HearSNPV-G4 and SeMNPV, and was most closely related to HearSNPV-G4. The nucleotide sequence identity between HearNPV-Au and HearSNPV-G4 genome was 99 %. The major differences were found in homologous regions (hrs) and baculovirus repeat ORFs (bro) genes. Five hrs and two bro genes were identified in the HearNPV-Au genome. All of the 134 ORFs identified in HearNPV-Au were also found in HearSNPV-G4, except the homologue of ORF59 (bro) in HearSNPV-G4. The sequence data strongly suggested that HearNPV-Au and HearSNPV-G4 belong to the same virus species. © 2013 Springer-Verlag Wien.
引用
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页码:595 / 601
页数:6
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