RNA interference inhibits yellow fever virus replication in vitro and in vivo

被引：0

作者：

Carolina C. Pacca

Adriana A. Severino

Adriano Mondini

Paula Rahal

Solange G. P. D’avila

José Antonio Cordeiro

Mara Correa Lelles Nogueira

Roberta V. M. Bronzoni

Maurício L. Nogueira

机构：

[1] Faculdade de Medicina de São José do Rio Preto–FAMERP,Laboratório de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias

[2] Universidade Estadual Paulista “Julio de Mesquita Filho”,Departamento de Biologia

[3] Faculdade de Medicina de São José do Rio Preto–FAMERP,Departamento de Patologia e Medicina Legal

[4] Faculdade de Medicina de São José do Rio Preto–FAMERP,Departamento de Epidemiologia e Saúde Coletiva

[5] Faculdade de Medicina de São José do Rio Preto–FAMERP,Laboratório de Microbiologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias

来源：

Virus Genes | 2009年 / 38卷

关键词：

RNAi; Yellow fever; Flaviviridae; Arboviruses;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

RNA interference (RNAi) is a process that is induced by double stranded RNA and involves the degradation of specific sequences of mRNA in the cytoplasm of the eukaryotic cells. It has been used as an antiviral tool against many viruses, including flaviviruses. The genus Flavivirus contains the most important arboviruses in the world, i.e., dengue (DENV) and yellow fever (YFV). In our study, we investigated the in vitro and in vivo effect of RNAi against YFV. Using stable cell lines that expressed RNAi against YFV, the cell lines were able to inhibit as much as 97% of the viral replication. Two constructions (one against NS1 and the other against E region of YFV genome) were able to protect the adult Balb/c mice against YFV challenge. The histopathologic analysis demonstrated an important protection of the central nervous system by RNAi after 10 days of viral challenge. Our data suggests that RNAi is a potential viable therapeutic weapon against yellow fever.

引用

页码：224 / 231

页数：7

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