Analysis of transcriptional activity of the Arabidopsis thaliana ornithine-delta-aminotransferase gene promoter

被引：0
作者：
S. V. Gerasimova
V. V. Gorelova
O. V. Dorogina
E. V. Zhmud
V. S. Koval
A. V. Romanova
A. V. Kochetova
V. K. Shumny
机构：
[1] Russian Academy of Sciences,Institute of Cytology and Genetics
[2] Novosibirsk State University,Department of Cytology and Genetics
[3] Russian Academy of Sciences,Central Siberian Botanical Garden
来源：
Russian Journal of Genetics | 2011年 / 47卷
关键词：
Transgenic Plant; Ornithine; Reporter Gene Activity; BamHI Restriction Site; Proline Biosynthesis;
D O I：
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摘要：
Cloning of the Arabidopsis thaliana genomic DNA fragment presumably corresponding to the promoter region of the ornithine-delta-aminotransferase (OAT) gene is reported. The reporter-gene construct, containing the Escherichia coli beta-glucouronidase gene under control of the OAT gene promoter was generated. The Nicotian tabacum SR1 transformants carrying this construct were obtained. It was demonstrated that under normal conditions, expression of the reporter gene was associated with the meristems and the zones of intensive shoot growth. Possible role of the OAT gene in nitrogen metabolism and shoot development is discussed.
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