Differential gene expression in duodenum of colored broiler chicken divergently selected for residual feed intake

被引：0
作者：
Prakash Anand
Saxena Vishesh Kumar
Kumar Ravi
Tomar Simmi
机构：
[1] College of Veterinary Science,Department of Livestock Farm Complex
[2] Rampura Phul,Division of Avian Genetics and Breeding, Central Avian Research Institute
[3] GADVASU,undefined
[4] Indian Council of Agricultural Research,undefined
[5] National institute of animal biotechnology,undefined
来源：
Tropical Animal Health and Production | 2021年 / 53卷
关键词：
Residual feed intake; Feed efficiency; Differential gene expression; Microarray;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Feed constitutes about 70% of the total expenditure of poultry production. Maximizing the feed efficiency in juvenile period is essential to achieve low production cost. The efficiency of feed utilization was measured by RFI (residual feed intake) by calculating the difference between an individual animal’s observed and its expected feed intake. The expression of genes influencing low and high RFI is required to know the basic molecular mechanism influencing feed efficiency. The present study aimed to estimate the RFI (0–5 week) in a population of indigenously developed colored broiler sire line chicken. The duodenum sample of high and low-RFI broiler chicken was used for microarray analysis. Duodenum exhibited 1030 differentially expressed genes after analysis. Out of total DEGs, 461 genes were downregulated and 569 were upregulated. The fold change of differentiallly expressed genes varies from − 162.6 to 1549.28. A subset of genes was validated by qRT-PCR and results were correlated well with microarray data. In functional annotation study of DEGs, 89 biological processes, 30 cellular components, and 29 molecular functions were identified. Study of the important differentially expressed genes and the related molecular pathways in the population may hold the potential for future breeding strategies for augmenting feed efficiency.
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