Growth and differentiation of periodontal ligament-derived cells in serum-free defined culture

被引：0

作者：

Okamoto T. ^{[1
,6
]}

Yatsuzuka N. ^{[2
]}

Tanaka Y. ^{[1
]}

Kan M. ^{[4
]}

Yamanaka T. ^{[2
]}

Sakamoto A. ^{[4
]}

Takata T. ^{[3
]}

Akagawa Y. ^{[2
]}

Sato G.H. ^{[5
]}

Sato J.D. ^{[5
]}

Takada K. ^{[1
]}

机构：

[1] Dept. Oral and Maxillofacial Surg. I, School of Dentistry, Hiroshima University

[2] Department of Prosthetic Dentistry I, School of Dentistry, Hiroshima University

[3] Department of Oral Pathology, School of Dentistry, Hiroshima University

[4] Ctr. for Cancer Biol. and Nutrition, Inst. of Bioscience and Technology, Texas A and M University, Houston

[5] W. Alton Jones Cell Science Center, Lake Placid, NY

[6] Dept. Oral and Maxillofacial Surg. I, School of Dentistry, Hiroshima University, Minami-ku, Hiroshima 734, 1-2-3, Kasumi

来源：

In Vitro Cellular & Developmental Biology - Animal | 1997年 / 33卷 / 4期

关键词：

alkaline phosphatase; FGF-1; FGF-2; FGFR-; 1; periodontal ligament cells; serum-free culture;

D O I：

10.1007/s11626-997-0051-0

中图分类号：

学科分类号：

摘要：

We have developed a serum-free medium for the growth and differentiation of periodontal ligament-derived cells (PLC). In addition, the expression of both fibroblast growth factor (FGF) and FGF receptor (FGFR) in the PLC was investigated by immunohistochemical examination, heparin affinity chromatography (HAC), and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Optimal growth of the cells was achieved in Iscove's modified Dulbecco's medium supplemented with insulin, transferrin 2- mercaptoethanol, 2-ethanolamine, sodium selenite, and oleic acid in type-I collagen-coated dishes. Both FGF-1 and FGF-2 stimulated cell growth and inhibited differentiation as measured by inhibition of alkaline phosphatase activity of the cells. An immunohistochemical analysis of FGF-1 and FGF-2 revealed that immunoreactive FGF-1 and FGF-2 were detected predominantly in the cytoplasm of growing cells. In addition, perinuclear FGF-1 staining and nuclear FGF-2 staining were observed in the same growing cells. In contrast, a faint diffuse staining of FGF-1 and FGF-2 was detected in cytoplasm of the confluent differentiated cells. The 2.15 M NaCl eluate from HAC of the cell extracts exhibited growth-promoting activities for the PLC, and it also stimulated the growth of human umbilical vein-derived endothelial cells and inhibited binding of [125I]-FGF to its receptors, indicating the cells produced FGFs or FGF-like growth factors. RT-PCR analysis revealed that the cells expressed FGFR-1 mRNA but not mRNAs for FGFR-2, FGFR-3 and FGFR-4 mRNA. These results suggest that the FGF-FGFR-1 system plays an important role in the growth and differentiation of periodontal ligament-derived cells.

引用

页码：302 / 309

页数：7

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