The promoter of aBrassica napus polygalacturonase gene directs pollen expression ofβ-glucuronidase in transgenicBrassica plants

被引：12
作者：
H. P. Hong
J. L. Gerster
R. S. S. Datla
D. Albani
G. Scoles
W. Keller
L. S. Robert
机构：
[1] National Research Council,Plant Biotechnology Institute
[2] Central Experimental Farm,Eastern Cereal and Oilseed Research Centre
[3] University of Saskatchewan,Department of Crop Science and Plant Ecology
[4] University of Pavia,Department of Genetics and Microbiology
来源：
Plant Cell Reports | 1997年 / 16卷 / 6期
关键词：
Pollen; Polygalacturonase; Promoter; Transformation; GUS;
D O I：
10.1007/BF01146776
中图分类号：
学科分类号：
摘要：
A 647-bp 5′-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed inBrassica napus pollen was fused to theβ-glucuronidase (GUS) marker gene. This fusion construct was introduced intoB. napus plants viaAgrobacterium tumefaciens transformation. Analysis of the transgenicB. napus plants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation.
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页码：373 / 378
页数：5
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