In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence

被引：0

作者：

Chunying Chen

Peiqun Zhang

Zhifang Chai

Guangcheng Li

Yuying Huang

机构：

[1] Institute of High Energy Physics,Laboratory of Nuclear Analytical Techniques

[2] Chinese Academy of Sciences,Synchrotron Radiation Laboratory

[3] Institute of High Energy Physics,undefined

[4] Chinese Academy of Sciences,undefined

来源：

Science in China Series A: Mathematics | 2000年 / 43卷

关键词：

synchrotron radiation; X-ray fluorescence analysis; analysis; metalloprotein, electrophoreticseparation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A method using synchrotron radiation X-ray fluorescence analysis toin situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak III in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique forin situ analysis of trace elements in protein bands.

引用

页码：88 / 92

页数：4

共 17 条

[1]

Laemmli U. K(1970)Cleavage of structural proteins during the assembly of the head of Bacteriophage T4 Nature 227 680-680

[2]

Chen . Y.(1999)Subeellular distribution of 25 elements in the human liver by neutron activation analysis Anal. Chem. (in Chinese) 27 278-278

[3]

Zhang P. Q.(1999)Subeellular distribution of Al, Cu, Mg, Mn and other elements in the human liver FreseniusJ. Anal. Chem. 363 512-512

[4]

Lu X. L.(1999)Subeellular distribution of selenium and Se-containing proteins in human liver Bioehimica et Biophysica Acta 1427 205-205

[5]

Chen C. Y.(1984)Elemental analysis of samples of biological origin relative to their protein content by means of charged particle bombardment Anal. Biochem. 139 418-418

[6]

Zhang P. Q.(1990)Location and quantification of metal ions in enzymes combining polyacrylmide gel electrophoresis and partical-induced X-ray emission Biol. Trace Element Res. 26 93-93

[7]

Lu X. L.(1996)Ion beam analysis of metalloproteins NIM Physics Res. B 109 234-234

[8]

Chen C. Y.(undefined)undefined undefined undefined undefined-undefined

[9]

Zhang P. Q.(undefined)undefined undefined undefined undefined-undefined

[10]

Hou X. L.(undefined)undefined undefined undefined undefined-undefined

← 1 2 →