Effects of carboxymethyl-chitosan on wound healing in vivo and in vitro

被引：22

作者：

Peng S. ^{[1
]}

Liu W. ^{[1
]}

Han B. ^{[1
]}

Chang J. ^{[1
]}

Li M. ^{[1
]}

Zhi X. ^{[1
]}

机构：

[1] College of Marine Life Sciences, Ocean University of China

来源：

Journal of Ocean University of China | 2011年 / 10卷 / 4期

关键词：

carboxymethyl-chitosan; cytokines; fibroblasts; macrophages; wound healing;

D O I：

10.1007/s11802-011-1764-y

中图分类号：

学科分类号：

摘要：

Effects of carboxymethyl-chitosan with different molecular weights on wound healing were investigated in vivo and in vitro. A second degree burn model was performed in rats and the accelerative effects of carboxymethyl-chitosan on wound repair were observed. Contents of transforming growth factor (TGF)-β1, interleukin(IL)-6 and matrix metalloproteinase (MMP)-1 in wounds were determined by enzyme-linked immunosorbent assay (ELISA). In vitro study evaluated the influence of carboxymethyl-chitosan on cytokines secretion of fibroblasts and macrophages. In vivo results showed that carboxymethyl-chitosan effectively accelerated the wound healing process in burned rats (P<0.05). Levels of TGF-β1, IL-6 and MMP-1 in carboxymethyl-chitosan groups were significantly elevated, compared with control group (P<0.05). In vitro results indicated that carboxymethyl-chitosan significantly promoted the proliferation of fibroblasts and stimulated its IL-8 and IL-10 secretion at different incubation time, but it did not affect collagen secretion of fibroblasts. Carboxymethyl-chitosan enhanced phagocytosis ability of macrophages, and increased its tumor necrosis factor (TNF)-α secretion. In conclusion, carboxymethyl-chitosan promoted wound healing by activating macrophages, accelerating fibroblasts growth and exerting considerable effects on the secretion of a series of cytokines. © 2011 Science Press, Ocean University of China and Springer-Verlag Berlin Heidelberg.

引用

页码：369 / 378

页数：9

共 47 条

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