Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids

被引：0
作者：
Lorenzo Caputi
Jakob Franke
Kate Bussey
Scott C. Farrow
Ivo Jose Curcino Vieira
Clare E. M. Stevenson
David M. Lawson
Sarah E. O’Connor
机构：
[1] Max Planck Institute of Chemical Ecology,
[2] Department of Natural Product Biosynthesis,undefined
[3] Leibniz University Hannover,undefined
[4] Centre for Biomolecular Drug Research,undefined
[5] John Innes Centre,undefined
[6] Department of Biological Chemistry,undefined
[7] Norwich Research Park,undefined
[8] Laboratorio de Ciencias Quimicas-UENF-Campos dos Goytacazes-RJ,undefined
来源：
Nature Chemical Biology | 2020年 / 16卷
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摘要：
Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity.
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页码：383 / 386
页数：3
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