Derivation of human embryonic stem cells in defined conditions

被引：0
|
作者：
Tenneille E Ludwig
Mark E Levenstein
Jeffrey M Jones
W Travis Berggren
Erika R Mitchen
Jennifer L Frane
Leann J Crandall
Christine A Daigh
Kevin R Conard
Marian S Piekarczyk
Rachel A Llanas
James A Thomson
机构：
[1] WiCell Research Institute,Department of Obstetrics and Gynecology
[2] PO Box 7365,The Department of Anatomy
[3] The Genome Center of Wisconsin,undefined
[4] The Wisconsin National Primate Research Center,undefined
[5] University of Wisconsin-Madison,undefined
[6] University of Wisconsin-Madison Medical School,undefined
[7] University of Wisconsin-Madison Medical School,undefined
来源：
Nature Biotechnology | 2006年 / 24卷
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摘要：
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
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页码：185 / 187
页数：2
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