Differential expression of VAMP2/synaptobrevin-2 after antidepressant and electroconvulsive treatment in rat frontal cortex

被引：41

作者：

Yamada M. ^{[1
]}

Takahashi K. ^{[1
]}

Tsunoda M. ^{[1
]}

Nishioka G. ^{[2
]}

Kudo K. ^{[2
]}

Ohata H. ^{[1
]}

Kamijima K. ^{[2
]}

Higuchi T. ^{[3
]}

Momose K. ^{[1
]}

Yamada M. ^{[1
]}

机构：

[1] Department of Pharmacology, Showa University, School of Pharmaceutical Sciences, Tokyo

[2] Department of Psychiatry, Showa University School of Medicine, Tokyo

[3] Kohnodai Hospital, National Ctr. of Neurol./Psychiatry, Chiba

[4] Department of Psychiatry, Showa Univ. Karasuyama Hospital, Setagaya, Tokyo 157-8577

来源：

The Pharmacogenomics Journal | 2002年 / 2卷 / 6期

基金：

日本学术振兴会;

关键词：

Depression; Differential cloning; Gene expression; Microarray; SSRI;

D O I：

10.1038/sj.tpj.6500135

中图分类号：

学科分类号：

摘要：

The biological basis for the therapeutic mechanisms of depression is still unknown. We have previously performed expressed-sequence tag (EST) analysis to identify some molecular machinery responsible for antidepressant effect. Then, we developed our original cDNA microarray, on which cDNA fragments identified as antidepressant-related genes/ESTs were spotted. In this study, with this microarray followed by Western blot analysis, we have demonstrated the induction of vesicle-associated membrane protein 2 (VAMP2/synaptobrevin-2) in rat frontal cortex not only after chronic antidepressant treatment, but also after repated electroconvulsive treatment. On the other hand, expression of SNAP-25 and syntaxin-1 was not changed by these treatments. These components make a soluble N- ethylmaleimide-sensitive fusion protein attachment protein receptor complex with VAMP2 and mediate the synaptic vesicle docking/fusion machiner. In conclusion, it is suggested that VAM2/synaptobrevin-2 plays important roles in the antidepressant effects. Our results may contribute to a novel model for the therapeutic mechamism of depression and new molecular targets for the development therapeutic agents.

引用

页码：377 / 382

页数：5

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