Epitheliotrophic capacity of serum and plasma eyedrops. Influence of centrifugation

被引：13

作者：

Herminghaus P. ^{[1
]}

Geerling G. ^{[1
,5
]}

Hartwig D. ^{[2
]}

Wedel T. ^{[3
]}

Dibbelt L. ^{[4
]}

机构：

[1] Klinik für Augenheilkunde, Univ. Klin. Schleswig-Holstein, Campus Lübeck

[2] Inst. Immunol. und Transfusionsmed., Univ. Klin. Schleswig-Holstein, Campus Lübeck

[3] Institut für Anatomie, Universität Lübeck

[4] Institut für klinische Chemie, Univ. Klin. Schleswig-Holstein, Campus Lübeck

[5] Klinik für Augenheilkunde, Univ. Klin. Schleswig-Holstein, Campus Lübeck, 23538 Lübeck

来源：

Der Ophthalmologe | 2004年 / 101卷 / 10期

关键词：

Cell culture; Cornea; Epithelium; Production; Serum;

D O I：

10.1007/s00347-003-0979-8

中图分类号：

学科分类号：

摘要：

Background. Serum eyedrops are a new modality for the treatment of ocular surface disorders. We examined the influence of the preparation of blood products in a cell culture model and compared it with plasma. Material and methods. Serum and plasma were obtained from full blood often healthy volunteers and centrifuged at 500 and 3000 G. EGF, PDGF, TGF-β1, fibronectin, and vitamin A were quantified by means of ELISA and HPLC. Cultures of human corneal epithelial cells were incubated with the four blood products in dose-response experiments and the intracellular ATP quantified. Results. EGF, PDGF, and vitamin A were present in serum in significantly higher concentrations than in plasma. The concentration of fibronectin was not influenced by the preparation. Support of proliferation was best by 25% platelet-poor serum. Serum supported the differentiation and migration of epithelial cells better than plasma. Conclusion. The biochemical character of serum eyedrops is determined by the parameters chosen to produce the blood product. Plasma does not seem to offer an epitheliotrophic capacity equivalent to serum eyedrops. Their production should be optimized before any meaningful randomized controlled clinical trial can be attempted.

引用

页码：998 / 1005

页数：7

共 32 条

[1]

Araki A., Ohashi Y., Sasabe T., Hayashi K., Watanabe H., Tano Y., Handa H., An SV-40-immortalized human corneal epithelial cell line and its characterization, Invest Ophthalmol Vis Sci, 36, pp. 614-621, (1995)

[2]

Barton K., Nava A., Monroy D.C., Pflugfelder S.C., Cytokines and tear function in ocular surface disease, Adv Exp Med Biol, 438, pp. 461-469, (1998)

[3]

Pilcher B.K., Dumin H.A., Sudbeck B.D., Krane S.M., Welgus H.G., Parks W.C., The activity of collagenase-1 is required for keratinocyte migration on a type 1 collagen matrix, J Cell Biol, 137, pp. 1445-1457, (1997)

[4]

Collins M.K., Perkins G.R., RodriguezTarduchy G., Nieto M.A., Lopez Rivas A., Growth factors as survival factors: Regulation of apoptosis, Bioassays, 16, pp. 133-138, (1994)

[5]

Ebner S., You L., Volcker H.E., Kruse F.E., Effekt von autologem serum auf die heilung nicht-infektiöser hornhautulzera und expression von wachstumsfaktor-rezeptoren in der kornea, Ophthalmologe, 98, (2001)

[6]

Ferreira De Souza R., Kruse F.E., Seitz B., Autologes serum bei sonst therapieresistenten hornhautepitheldefekten - Prospektive studie an den ersten 70 augen, Klin Monatsbl Augenheilkd, 218, pp. 720-726, (2001)

[7]

Fox R.I., Chan R., Michelson J.B., Belmont J.B., Michelson P.E., Beneficial effect of artificial tears made with autologous serum in patients with keratoconjunctivitis sicca, Arthritis Rheum, 27, pp. 459-461, (1984)

[8]

Fredj-Reygrobellet D., Plouet J., Delazre T., Baudouin C., Bourret F., Lapalus P., Effects of aFGF and bFGF on wound healing in rabbit corneas, Curr Eye Res, 6, pp. 1205-1209, (1987)

[9]

Fukuda M., Fullard R.J., Willcox M.D., Et al., Fibronectin in the tear film, Invest Ophthalmol Vis Sci, 37, pp. 459-467, (1996)

[10]

Geerling G., Daniels J.T., Dart J.K., Cree I.A., Khaw P.T., Toxicity of natural tear substitutes in a fully defined culture model of human corneal epithelial cells, Invest Ophthalmol Vis Sci, 42, pp. 948-956, (2001)

← 1 2 3 4 →