Caffeic Acid Inhibits Chromium(VI)-Induced Oxidative Stress and Changes in Brush Border Membrane Enzymes in Rat Intestine

We have previously shown that a single oral dose of potassium dichromate results in a decrease in the activities of several brush border membrane enzymes, produces oxidative stress, and alters the activities of several antioxidant enzymes in the small intestine of rats. In the present study, we have investigated the effect of treatment with the dietary antioxidant caffeic acid on potassium dichromate-induced biochemical changes in the rat intestine. Adult male Wistar rats were randomly divided into four groups: control, potassium dichromate alone, caffeic acid alone, and potassium dichromate + caffeic acid. Administration of a single oral dose of potassium dichromate alone (100 mg/kg body mass) led to a decrease in the activities of brush border membrane enzymes, increase in lipid peroxidation, decrease in sulfhydryl groups, and changes in the activities of several antioxidant enzymes. Two oral doses of caffeic acid (each of 250 mg/kg body mass) greatly attenuated the potassium dichromate-induced changes in all these parameters, but the administration of caffeic acid alone had no effect. Thus, caffeic acid is an effective agent in reducing the effects of potassium dichromate on the intestine and could prove to be useful in alleviating the toxicity of chromium(VI) compounds.