Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India

被引：0
作者：
Jaiswal Namrata
R. K. Saritha
D. Datta
M. Singh
R. S. Dubey
A. B. Rai
M. Rai
机构：
[1] Indian Institute of Vegetable Research,Department of Biochemistry
[2] P/O Jakhini (Shahanshapur),undefined
[3] Banaras Hindu University,undefined
来源：
Indian Journal of Virology | 2010年 / 21卷
关键词：
Begomovirus; ToLCPMV; Pumpkin; Betasatellite;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Pumpkin cultivation in India is affected by severe incidence of a yellow vein mosaic disease. Tomato leaf curl New Delhi virus and Squash leaf curl China virus are known to be associated with this disease in India. We were able to identify a third begomovirus—Tomato leaf curl Palampur virus (ToLCPMV), from pumpkin showing typical symptoms of the disease at Varanasi based on the sequence of complete DNA-A genome of the virus. The complete DNA-A sequence of the virus shared more than 99% sequence identity with other ToLCPMV isolates available in the GenBank and clustered with them in the phylogenetic analysis. This betasatellite amplified from the same infected sample has been identified as Pepper leaf curl betasatellite (PepLCB) which also infects chilli in India. There was 92% sequence identity between the two isolates. This is the first report of natural infection of ToLCPMV on pumpkin and association of PepLCB with yellow vein mosaic disease of pumpkin in India.
引用
收藏
页码：128 / 132
页数：4
相关论文
共 99 条
[11]  
Mansoor S(2006)Identification of a novel circular single stranded DNA associated with cotton leaf curl disease in Pakistan Trends Plant Sci 11 209-212
[12]  
Bedford ID(2003)Geminivirus disease complexes: the threat is spreading J Plant Pathol 85 63-65
[13]  
Rishi N(2007)Pumpkin yellow vein mosaic virus: a novel begomovirus infecting cucurbits EPPO Bull 37 412-419
[14]  
Siwath SS(2003)Pumpkin yellow vein mosaic disease is caused by two distinct begomoviruses: complete viral sequences and comparative transmission by an indigenous Ann Appl Biol 142 323-331
[15]  
Zafar MY(2009) and the introduced B-biotype J Virol 83 9347-9355
[16]  
Abdel-Salam AM(1993)Characterization of pumpkin yellow vein mosaic virus Nucleic Acid Res 21 3328-394
[17]  
Markham PG(2005)Maintenance of an old world betasatellite by a new world helper begomovirus and possible rapid adaptation of the betasatellite Annu Rev Phytopathol 43 361-3172
[18]  
Briddon RW(2008)Mediprep method for isolation of DNA from plant samples with high content of polyphenolics J Gen Virol 89 3165-228
[19]  
Brown JK(2008)Exploiting chinks in the plant’s armor: evolution and emergence of geminiviruses J Phytopathol 156 222-1599
[20]  
Moriones E(2007)Replication promiscuity of DNA–β satellites associated with monopartitte begomoviruses; deletion mutagenesis of the Ageratum yellow vein virus DNA–β satellite localises sequences involved in replication Mol Biol Evol 24 1596-4680
12345678910