Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients

被引：0
作者：
Marijke Grau
Max Jerke
Elie Nader
Alexander Schenk
Celine Renoux
Bianca Collins
Thomas Dietz
Daniel Alexander Bizjak
Philippe Joly
Wilhelm Bloch
Philippe Connes
Aram Prokop
机构：
[1] German Sport University Cologne,
[2] Department of Molecular and Cellular Sports Medicine,undefined
[3] University of Lyon,undefined
[4] University Claude Bernard Lyon 1,undefined
[5] Interuniversity Laboratory of Human Movement Biology EA7424,undefined
[6] “Vascular Biology and Red Blood Cell” team,undefined
[7] Laboratory of Excellence “GR-Ex”,undefined
[8] East Biology Centre,undefined
[9] UF “Biochemistry of Red Blood Cell Disease”,undefined
[10] Academic Hospital of Lyon,undefined
[11] HCL,undefined
[12] Children’s Hospital Amsterdamer Straße Cologne; Clinic for Children and Youth Medicine,undefined
[13] Paediatric Oncology/Haematology,undefined
来源：
Scientific Reports | / 9卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subjects reached the first ventilatory threshold (VT 1). Blood was sampled at rest and at VT 1. At rest, free haemoglobin level was higher and RBC count, haemoglobin and haematocrit were lower in SCA compared to AA. RBC deformability was lower in SCA. Exercise had no effect on the tested parameters. RBC NO level was higher in SCA compared to AA at rest and significantly decreased after exercise in SCA. This might be related to a reduction in RBC-NO synthase (RBC-NOS) activation which was only observed in SCA after exercise. Free radical levels were higher in SCA at rest but concentration was not affected by exercise. Marker for lipid peroxidation and antioxidative capacity were similar in SCA and AA and not affected by exercise. In conclusion, a single acute submaximal bout of exercise has no deleterious effects on RBC deformability or oxidative stress markers in SCA, and seems to modulate RBC-NOS signalling pathway.
引用
收藏
相关论文
共 106 条
[11]  
Chasis JA(2012)Doubts concerning functional endothelial nitric oxide synthase in human erythrocytes Blood. 119 1322-1219
[12]  
Kleinbongard P(1996)Erythrocytes may synthesize their own nitric oxide Am. J. Hypertens. 9 1214-449
[13]  
Grau M(2001)Purification and properties of insulin-activated nitric oxide synthase from human erythrocyte membranes Arch. Physiol. Biochem. 109 441-15054
[14]  
Bor-Kucukatay M(2013)Arginase regulates red blood cell nitric oxide synthase and expoert of cardioprotective nitric oxide bioactivity Proc. Natl. Acad. Sci. USA 110 15049-103
[15]  
Wenby RB(2009)Intensive exercise induces changes of endothelial nitric oxide synthase pattern in human erythrocytes Nitric Oxide. 20 95-736
[16]  
Meiselman HJ(2012)Moderate exercise promotes human RBC-NOS activity, NO production and deformability through Akt kinase pathway PloS One. 7 e45982-53
[17]  
Baskurt OK(2015)High red blood cell nitric oxide synthase activation is not associated with improved vascular function and red blood cell deformability in sickle cell anaemia Br J Haematol. 168 728-141
[18]  
Böhmer A(2016)Red blood cell nitric oxide synthase modulates red blood cell deformability in sickle cell anemia Clin Hemorheol Microcirc. 64 47-199
[19]  
Beckmann B(2010)New fundamental and applied mechanisms in exercise hemorheology Clin. Hemorheol. Microcirc. 45 131-31
[20]  
Sandmann J(2013)Exercise hemorheology: classical data, recent findings and unresolved issues Clin. Hemorheol. Microcirc. 53 187-366
12345678910