Aqueous-phase biofunctionalized NH2-MIL-53(Al) MOF for biosensing applications

被引：0
作者：
Rajit Sikka
Pawan Kumar
Jechan Lee
Christian Sonne
机构：
[1] Central University of Jammu,Department of Nano Sciences and Materials
[2] Ajou University,Department of Environmental and Safety Engineering & Department of Energy Systems Research
[3] Aarhus University,Department of Bioscience, Arctic Research Centre (ARC), Faculty of Science and Technology
来源：
Journal of Porous Materials | 2022年 / 29卷
关键词：
Amino based metal–organic framework; Pendent group; Bioconjugation; BSA photoluminescence quenching;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Here, we synthesize an amino-based metal–organic framework (MOF) encompassing NH2-MIL-53(Al) in water using chemical stirring at room temperature. The pendent group on synthesized NH2-MIL-53(Al) was activated using MES buffer. Afterward, biofunctionalization of MOF have been established through conjugation with anti-bovine serum albumin (anti-BSA) using EDC-NHS. Carbodiimide (–CONH2) functional group in Anti-BSA–NH2-MIL-53(Al) conjugate was confirmed through spectroscopic analysis. Importantly, Anti-BSA conjugated MOF was utilized for BSA optical sensing on basis of photoluminescence quenching mechanism in aqueous media. The limit of detection (LOD) was found 1 ppb for BSA. Expectantly, novel anti-BSA-conjugated NH2-MIL-53(Al) will be recognized as a novel material for biomedical applications such as diagnosis, biosensors and drug delivery in near future.
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页码：515 / 522
页数：7
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