Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rich. an endemic orchid to Western Ghats

被引：21

作者：

Abraham S. ^{[2
]}

Augustine J. ^{[1
]}

Thomas T.D. ^{[1
]}

机构：

[1] Post Graduate and Research Department of Botany, St. Thomas College, Palai, Kottayam, Kerala, Arunapuram (PO), PIN-686574, Kottayam (DT)

[2] Department of Botany, Bharathiar University, Coimbatore, Tamilnadu

来源：

Physiology and Molecular Biology of Plants | 2012年 / 18卷 / 3期

关键词：

Coelogyne nervosa; Epiphytic orchid; Micropropagation; Seedlings; Tissue culture;

D O I：

10.1007/s12298-012-0118-6

中图分类号：

学科分类号：

摘要：

Coelogyne nervosa is an epiphytic orchid endemic to Western Ghats, South India. The mature seeds of C. nervosa were cultured on 1/2 MS (Murashige and Skoog), MS, Kn (Knudson) and VW (Vacin and Went) media to evaluate the seed germination response. Of the four basal media used, MS medium supported maximum seed germination. Further experiments to enhance seed germination were done on MS medium supplemented with various concentrations (10, 20, 30 and 40 %) of coconut water (CW). Thirty percent CW gave the highest response in terms of percent seed germination (96), fresh weight (7. 2 mg/seedling) and protocorm length (15. 2 mm). Since CW containing medium did not support further seedling growth, each seedling was isolated and cultured on MS medium supplemented with either BA (6-benzylaminopurine) or Kin (kinetin) alone (1. 0-4. 0 mg/l each) or in combination with NAA (1-naphthaleneacetic acid; 0. 2-1. 0 mg/l). Maximum growth was observed on MS medium supplemented with BA (3. 0 mg/l) and NAA (0. 5 mg/l). On this medium, the seedlings reached an average length of 3. 6 cm with 2. 8 well expanded green leaves per seedling. Similarly optimum, healthy, white root induction (3. 3 roots/seedlings) was also observed on the same medium. The rooted seedlings were successfully transplanted to pots with 91 % success. The 2-year-old tissue culture derived plants produced normal flowers and fruits. © 2012 Prof. H.S. Srivastava Foundation for Science and Society.

引用

页码：245 / 251

页数：6

共 28 条

[1]

Abraham A., Vatsala P., Introduction to orchids with illustrations and descriptions of 150 South Indian orchids, Tropical botanical graden and research Institute, pp. 285-287, (1981)

[2]

US department of agriculture, Floriculture crops 2007 summary, (2008)

[3]

Bhadra S.K., Hossain M.M., In vitro germination and micropropagation of Geodorum densiflorum (Lam.) Schltr., an endangered orchid species, Plant Tissue Cult, 13, pp. 65-171, (2003)

[4]

Chugh S., Guha S., Rao U.I., Micropropagation of orchids: a review on the potential of different explants, Sci Hortic, 122, pp. 507-520, (2009)

[5]

Duncan D.B., Multiple range and multiple F test, Biometrics, 11, pp. 1-42, (1955)

[6]

Hey G.L., Hey M.G., Raising rare orchids from seeds, Proc. 5th World Orchid Conf, pp. 35-38, (1966)

[7]

Hossain M.M., Asymbiotic seed germination and in vitro seedling development of Epidendrum ibaguense Kunth. (Orchidaceae), Afr J Biotechnol, 7, pp. 3614-3619, (2008)

[8]

Khampa S., Wangsomnuk P., Wangsomnuk P., Factors affecting seed germination of Grammatophylum speciosum cultured in vitro, AsPac J Mol Biol Biotechnol, 18, pp. 193-197, (2010)

[9]

Khasim S.M., Ramudu J., Genetic diversity in Coelogyne nervosa R. Rich., an endemic orchid from Southern India, Plant Canada, 2011, Proceedings of the joint meeting of the Canadian society of agronomy, (2011)

[10]

Kitsaki C.K., Zygouraki S., Ziobora M., Kintzios S., In vitro germination, protocorm formation and plantlet development of mature versus immature seeds from several Ophrys species (Orchidaceae), Plant Cell Rep, 23, pp. 284-290, (2004)

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