Hydrogen peroxide modulates expression of surface antigens by human umbilical vein endothelial cells in vitro

被引:7
|
作者
Kudryavtsev I.V. [1 ,2 ,3 ]
Garnyuk V.V. [4 ]
Nadeev A.D. [5 ]
Goncharov N.V. [4 ,6 ]
机构
[1] Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, ul. Acad. Pavlova, 12, St. Petersburg
[2] Far-Eastern Federal University, Vladivostok
[3] St. Petersburg State University, St. Petersburg
[4] Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency, St. Petersburg
[5] Institute of Cell Biophysics, Russian Academy of Sciences, ul. Instituskaya, 3, Pushchino, Moscow Oblast
[6] Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg
关键词
CD antigens; endothelial cells; expression; hydrogen peroxide;
D O I
10.1134/S1990747813050103
中图分类号
学科分类号
摘要
The aim of this study was to determine the immunological phenotype of human umbilical vein endothelial cells in culture and dynamics of expression of inducible CD molecules of the endothelial cell surface exposed to hydrogen peroxide (H2O2, 100-300 μM) in vitro. PMA was applied as a positive control. There was an insignificant increase (9-21%) of CD31 expression 3 h after the addition of H2O2to the culture medium, statistically significant decrease of CD309 expression upon exposure to 200 and 300 μM H2O2, whereas a day after the addition of H2O2we found a strongly pronounced dose-dependent increase of CD54 expression, as well as a moderate enhancement of the expression of all CD molecules. © 2014 Pleiades Publishing, Ltd.
引用
收藏
页码:97 / 102
页数:5
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