In silico characterization and transcriptomic analysis of nif family genes from Anabaena sp. PCC7120

被引：0
作者：
Shilpi Singh
Alok Kumar Shrivastava
机构：
[1] Banaras Hindu University,Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany
[2] Mahatma Gandhi Central University Bihar,Department of Botany
来源：
Cell Biology and Toxicology | 2017年 / 33卷
关键词：
Bioinformatics; CD search; Homology modeling; Docking; qRT; sp. PCC7120; Abiotic stresses;
D O I：
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中图分类号：
学科分类号：
摘要：
In silico approaches in conjunction with morphology, nitrogenase activity, and qRT-PCR explore the impact of selected abiotic stressor such as arsenic, salt, cadmium, copper, and butachlor on nitrogen fixing (nif family) genes of diazotrophic cyanobacterium Anabaena sp. PCC7120. A total of 19 nif genes are present within the Anabaena genome that is involved in the process of nitrogen fixation. Docking studies revealed the interaction between these nif gene-encoded proteins and the selected abiotic stressors which were further validated through decreased heterocyst frequency, fragmentation of filaments, and downregulation of nitrogenase activity under these stresses indicating towards their toxic impact on nitrogen fixation potential of filamentous cyanobacterium Anabaena sp. PCC7120. Another appealing finding of this study is even though having similar binding energy and similar interacting residues between arsenic/salt and copper/cadmium to nif-encoded proteins, arsenic and cadmium are more toxic than salt and copper for nitrogenase activity of Anabaena which is crucial for growth and yield of rice paddy and soil reclamation.
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页码：467 / 482
页数：15
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