Immuno-engineered organoids for regulating the kinetics of B-cell development and antibody production

被引:0
作者
Alberto Purwada
Ankur Singh
机构
[1] Meinig School of Biomedical Engineering,
[2] ,undefined
[3] Cornell University,undefined
[4] Sibley School of Mechanical and Aerospace Engineering,undefined
[5] Cornell University,undefined
来源
Nature Protocols | 2017年 / 12卷
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摘要
This protocol describes how to generate mouse 3D immune organoids consisting of germinal-center-like B cells in a gelatin matrix.
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页码:168 / 182
页数:14
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[1]  
Shi W(2015)Transcriptional profiling of mouse B cell terminal differentiation defines a signature for antibody-secreting plasma cells Nat. Immunol. 16 663-673
[2]  
Saylor C(2009)Monoclonal antibody-based therapies for microbial diseases Vaccine 27 G38-G46
[3]  
Dadachova E(2014)Novel tumor antigen-specific monoclonal antibody-based immunotherapy to eradicate both differentiated cancer cells and cancer-initiating cells in solid tumors Semin. Oncol. 41 685-699
[4]  
Casadevall A(2012)Antibody-based immunotherapy of cancer Cell 148 1081-1084
[5]  
Sabbatino F(2008)Immunotherapy of autoimmunity and cancer: the penalty for success Nat. Rev. Immunol. 8 970-976
[6]  
Weiner LM(2015)The generation of antibody-secreting plasma cells Nat. Rev. Immunol. 15 160-171
[7]  
Murray JC(2001)An essential role for BAFF in the normal development of B cells through a BCMA-independent pathway Science 293 2111-2114
[8]  
Shuptrine C(2014)Follicular dendritic cells: dynamic antigen libraries Nat. Rev. Immunol. 14 495-504
[9]  
Caspi R(1998)CD40 ligand and appropriate cytokines induce switching to IgG, IgA, and IgE and coordinated germinal center and plasmacytoid phenotypic differentiation in a human monoclonal IgM+ IgD+ B cell line J. Immunol. 160 2145-2157
[10]  
Nutt SL(2002)Human primary and memory cytotoxic T lymphocyte responses are efficiently induced by means of CD40-activated B cells as antigen-presenting cells: potential for clinical application Blood 99 3319-3325