Optimization of extraction of β-endoglucanase from the fermented bran of Aspergillus niger

被引：0
|
作者：
M. Subhosh Chandra
Buddolla Viswanath
B. Rajasekhar Reddy
机构：
[1] College of Natural Resources and Life Science,Department of Biotechnology
[2] Durban University of Technology,Department of Biotechnology, Center for Water and Waste
[3] Sri Krishnadevaraya University,water Technology
来源：
Indian Journal of Microbiology | 2010年 / 50卷
关键词：
Endoglucanase; Extraction; Solid state fermentation; Wheat bran;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A local isolate of Aspergillus niger was cultivated under optimal growth conditions on wheat bran in solid state fermentation. β-endoglucanase from fermented bran was separately extracted with different solvents to test recovery of enzyme. Among solvents tested, distilled water served the best leachate. Conditions were further optimized with this leachate. Two washes of fermented bran with the leachate for 30 min each under shaking conditions in a ratio of 1 g of wheat bran: 4 ml of distilled water together yielded maximum recovery of 16.7 U/g of wheat bran.
引用
收藏
页码：122 / 126
页数：4
相关论文
共 50 条
  • [1] Optimization of extraction of β-endoglucanase from the fermented bran of Aspergillus niger
    Chandra, M. Subhosh
    Viswanath, Buddolla
    Reddy, B. Rajasekhar
    INDIAN JOURNAL OF MICROBIOLOGY, 2010, 50 : S122 - S126
  • [2] Infusion of an endoglucanase and an endoxylanase from Aspergillus niger in soybean
    Varzakas, TH
    Arapoglou, D
    Israilides, CJ
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2005, 38 (03) : 239 - 247
  • [3] Uptake of endoglucanase and endoxylanase from Aspergillus niger by soybean seeds
    Varzakas, TH
    DEUTSCHE LEBENSMITTEL-RUNDSCHAU, 2001, 97 (12) : 465 - +
  • [4] Optimization of Carotenoid Pigment Extraction from Epicoccum nigrum Fermented Wheat Bran
    Kaur, Sawinder
    Panesar, Paramjit S.
    Gurumayum, Sushma
    Rasane, Prasad
    Kumar, Vikas
    INDUSTRIAL BIOTECHNOLOGY, 2021, 17 (02) : 100 - 104
  • [5] GENERAL AND KINETIC-PROPERTIES OF ENDOGLUCANASE FROM ASPERGILLUS-NIGER
    SINGH, A
    AGRAWAL, AK
    ABIDI, AB
    DARMWAL, NS
    FEMS MICROBIOLOGY LETTERS, 1990, 71 (1-2) : 221 - 224
  • [6] THE EFFECTS OF RICE BRAN FERMENTED WITH Aspergillus niger ON QUALITY OF PIG PRODUCT TO ENSURE FOOD SECURITY
    Najoan, Marie
    Bagau, Betty
    Wolayan, Fenny
    Anis, Selvie
    Rumokoy, Laurentius
    SCIENTIFIC PAPERS-SERIES D-ANIMAL SCIENCE, 2019, 62 (01): : 75 - 78
  • [7] Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation
    Julia Boggione, Maria
    Becher, Ramiro
    Farruggia, Beatriz
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2016, 7 : 118 - 126
  • [8] Parametric optimization of extracellular α-amylase recovery from the fermented bran of Aspergillus awamori MTCC 9997
    Kalaiarasi, K.
    Parvatham, R.
    AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, 2011, 5 (17): : 2474 - 2478
  • [9] IMPROVING THE EXTRACTION CONDITIONS OF ENDOGLUCANASE PRODUCED BY Aspergillus niger UNDER SOLID-STATE FERMENTATION
    Pirota, R. D. P. B.
    Miotto, L. S.
    Delabona, P. S.
    Farinas, C. S.
    BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, 2013, 30 (01) : 117 - 123
  • [10] Purification and properties of an endoglucanase from Aspergillus niger VTCC-F021
    Thi Hoa Pham
    Dinh Thi Quyen
    Ngoc Minh Nghiem
    TURKISH JOURNAL OF BIOLOGY, 2012, 36 (06) : 694 - 701
12345