Optimization of extraction of β-endoglucanase from the fermented bran of Aspergillus niger

被引：0
作者：
M. Subhosh Chandra
Buddolla Viswanath
B. Rajasekhar Reddy
机构：
[1] College of Natural Resources and Life Science,Department of Biotechnology
[2] Durban University of Technology,Department of Biotechnology, Center for Water and Waste
[3] Sri Krishnadevaraya University,water Technology
来源：
Indian Journal of Microbiology | 2010年 / 50卷
关键词：
Endoglucanase; Extraction; Solid state fermentation; Wheat bran;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A local isolate of Aspergillus niger was cultivated under optimal growth conditions on wheat bran in solid state fermentation. β-endoglucanase from fermented bran was separately extracted with different solvents to test recovery of enzyme. Among solvents tested, distilled water served the best leachate. Conditions were further optimized with this leachate. Two washes of fermented bran with the leachate for 30 min each under shaking conditions in a ratio of 1 g of wheat bran: 4 ml of distilled water together yielded maximum recovery of 16.7 U/g of wheat bran.
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页码：122 / 126
页数：4
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共 72 条
  • [1] Aristidou A.(2000)Metabolic engineering applications to renewable resource utilization Curr Opin Biotech 11 478-483
  • [2] Pentilla M.(2000)Treatment of recycled kraft pulps with J Biotech 78 39-48
  • [3] Oksanen T.(2002) hemicellulases and cellulases Microbiol Mol Biol Rev 66 506-577
  • [4] Pere J.(1995)Microbial cellulose utilization: Fundamentals and biotechnology Enzyme Microb Technol 17 942-946
  • [5] Paavilainen L.(2001)Enhanced cellulase production by a mutant of Trends in Biotech 19 172-177
  • [6] Buchert J.(2002)Biotechnology and utilization of biowaste as a resource for bioproduct development World J Microbiol Biotechnol 18 487-491
  • [7] Viikari L.(1999)Regulatory mutations affecting the synthesis of cellulase in Curr Sci 77 149-162
  • [8] Lynd L.R.(1982)Solid state fermentation for the production of Industrial enzymes Indian J Technol 20 476-480
  • [9] Weimer P.J.(1999)Recovery of amyloglucosidase from moldy bran J Environ Biol 20 235-239
  • [10] Van Zyl W.H.(2007)Cellulolytic activity of fungal cultures isolated from soil contaminated with effluents of cotton ginning industry Ind J Microbiol 47 323-328
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