Characterization of the Immunogenic and Antigenic Potential of Putative Lipoproteins from Leptospira interrogans

被引：0
作者：
Daiane D. Hartwig
Fabiana K. Seixas
Gustavo M. Cerqueira
Alan J. A. McBride
Odir A. Dellagostin
机构：
[1] UFPel,Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico
[2] Instituto Butantan,Laboratório de Biotecnologia Molecular II, Centro de Biotecnologia
[3] Instituto Gonçalo Moniz,Laboratório de Patologia e Biologia Molecular
[4] Fundação Oswaldo Cruz,Department of Microbiology
[5] Monash University,undefined
来源：
Current Microbiology | 2011年 / 62卷
关键词：
Recombinant Protein; Lyme Disease; Vaccine Candidate; Leptospirosis; Neisseria Meningitidis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The search for a vaccine capable of conferring heterologous protection, through the identification of conserved and cross-protective antigens, remains an ongoing priority in leptospirosis research. In the present study, an in silico analysis was used to identify potentially protective lipoproteins from Leptospira interrogans serovar Copenhageni. Eight putative lipoproteins were selected (LIC10009, LIC10054, LIC10091, LIC11058, LIC11567, LIC13059, LIC13305, and LIC20172), cloned and expressed in Escherichia coli and purified by affinity chromatography. The recombinant proteins were used to inoculate mice and the subsequent humoral immune response was evaluated by ELISA. Seven of the potential lipoproteins induced a significant IgG response. Furthermore, all of the recombinant proteins were recognized by antibodies present in the sera of severe leptospirosis patients. These putative lipoproteins exhibited potential for further evaluation as prospective vaccine candidates.
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页码：1337 / 1341
页数：4
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