Microbial based assay for specific detection of β-lactam group of antibiotics in milk

被引：0
|
作者：
Sougata Das
Naresh Kumar
Raghu Hirikyathanahalli Vishweswaraiah
Lopamudra Haldar
Manju Gaare
Vinai Kumar Singh
Anil Kumar Puniya
机构：
[1] National Dairy Research Institute,Dairy Microbiology Division
[2] The ICFAI University,undefined
来源：
Journal of Food Science and Technology | 2014年 / 51卷
关键词：
β-lactam; Induction; β-lactamase; Spore; Assay;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The spore forming Bacillus cereus (66) was screened for the induction of β-lactamase in presence of an inducer using iodometric assay. A significant induction in marker enzyme was observed in B. cereus 66 at maximum residual limit (MRL) of penicillin, ampicillin, cloxacillin, amoxicillin, cefalexin, and cephazolin belonging to β-lactam group of antibiotics. A microbial based assay, where enzyme induction was optimized at pH 7.0, temperature 30°C, and whey powder (0.25%) after 4 h of incubation. The spore based assay was tested with milk samples spiked with 6 different β-lactam antibiotics. The results were 100 and 83.33% in correlation with microbial receptor and inhibition based assay, respectively. Overall, spore based assay can be a useful and cost effective tool for the specific detection of β-lactam group of antibiotics in milk.
引用
收藏
页码：1161 / 1166
页数：5
相关论文
共 50 条
  • [21] Heat inactivation of β-lactam antibiotics in milk
    Zorraquino, M. A.
    Roca, M.
    Fernandez, N.
    Molina, M. P.
    Althaus, R.
    JOURNAL OF FOOD PROTECTION, 2008, 71 (06) : 1193 - 1198
  • [22] STUDIES ON DISC ASSAY METHODS FOR DETECTION OF ANTIBIOTICS IN MILK
    MARTH, EH
    HUSSONG, RV
    ALEXANDE.FJ
    JOURNAL OF MILK AND FOOD TECHNOLOGY, 1963, 26 (05): : 150 - &
  • [23] Development of an optical biosensor assay for detection of β-lactam antibiotics in milk using the penicillin-binding protein 2x
    Cacciatore, G
    Petz, M
    Rachid, S
    Hakenbeck, R
    Bergwerff, AA
    ANALYTICA CHIMICA ACTA, 2004, 520 (1-2) : 105 - 115
  • [24] The Swedish system for detection and separation of β-lactam antibiotics contaminated milk -: a practical approach
    Sternesjö, Å
    Johnsson, G
    MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL, 2003, 58 (1-2): : 68 - 69
  • [25] MICROBIAL RESISTANCE TO BETA-LACTAM ANTIBIOTICS
    WASHINGTON, JA
    MAYO CLINIC PROCEEDINGS, 1982, 57 (12) : 781 - 783
  • [26] Qualitative detection of tetracycline residues in milk with a luminescence-based microbial method:: The effect of milk composition and assay performance in relation to an immunoassay and a microbial inhibition assay
    Kurittu, J
    Lönnberg, S
    Virta, M
    Karp, M
    JOURNAL OF FOOD PROTECTION, 2000, 63 (07) : 953 - 957
  • [27] Development of a receptor-based microplate assay for the detection of beta-lactam antibiotics in different food matrices
    Lamar, Janine
    Petz, Michael
    ANALYTICA CHIMICA ACTA, 2007, 586 (1-2) : 296 - 303
  • [28] An amperometric affinity penicillin-binding protein magnetosensor for the detection of β-lactam antibiotics in milk
    Gamella, M.
    Campuzano, S.
    Conzuelo, F.
    Esteban-Torres, M.
    de las Rivas, B.
    Reviejo, A. J.
    Munoz, R.
    Pingarron, J. M.
    ANALYST, 2013, 138 (07) : 2013 - 2022
  • [29] Rapid Detection of Bacterial Resistance to β-Lactam Antibiotics with a Relay-Response Chemiluminescence Assay
    Ma, Zheng
    Liu, Runqiu
    Wang, Jie
    Yu, Tao
    Zou, Yingqiu
    Chen, Fangfang
    Cui, Cui
    Yang, Huixin
    Xie, Hexin
    ACS INFECTIOUS DISEASES, 2024, 10 (06): : 1970 - 1979
  • [30] Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics
    Boehle, Katherine E.
    Carrell, Cody S.
    Caraway, Joseph
    Henry, Charles S.
    ACS SENSORS, 2018, 3 (07): : 1299 - 1307
12345