Enhanced tolerance of transgenic potato plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against oxidative stress and high temperature

被引：0
作者：
Li Tang
Suk-Yoon Kwon
Sun-Hyung Kim
Jin-Seog Kim
Jung Sup Choi
Kwang Yun Cho
Chang K. Sung
Sang-Soo Kwak
Haeng-Soon Lee
机构：
[1] Korea Research Institute of Bioscience and Biotechnology (KRIBB),Environmental Biotechnology Research Center
[2] Bioorganic Science Division,Biofunction Research Team
[3] Korea Research Institute of Chemical Technology (KRICT),Department of Food Biotechnology
[4] Chungnam National University,undefined
[5] Yuseong,undefined
来源：
Plant Cell Reports | 2006年 / 25卷
关键词：
Antioxidant enzyme; Environmental stress; Molecular breeding; Potato; Transgenic plant;
D O I：
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中图分类号：
学科分类号：
摘要：
Oxidative stress is a major damaging factor for plants exposed to environmental stresses. In order to develop transgenic potato plants with enhanced tolerance to environmental stress, the genes of both Cu/Zn superoxide dismutase and ascorbate peroxidase were expressed in chloroplasts under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SSA plants). SSA plants showed enhanced tolerance to 250 μM methyl viologen, and visible damage in SSA plants was one-fourth that of non-transgenic (NT) plants that were almost destroyed. In addition, when SSA plants were treated with a high temperature of 42°C for 20 h, the photosynthetic activity of SSA plants decreased by only 6%, whereas that of NT plants decreased by 29%. These results suggest that the manipulation of the antioxidative mechanism of the chloroplasts may be applied in the development of industrial transgenic crop plants with increased tolerance to multiple environmental stresses.
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页码：1380 / 1386
页数：6
相关论文
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