Enzyme mass-transfer coefficient in aqueous two-phase systems using static mixer extraction column

被引：0

作者：

K. Rostami

M. Alamshahi

机构：

[1] No. 71 Forsat Street,

[2] Biotechnology Center,undefined

[3] Iranian Research Organization for Science and Technology,undefined

[4] Tehran-15819,undefined

[5] Iran,undefined

来源：

Bioprocess and Biosystems Engineering | 2002年 / 25卷

关键词：

Enzyme mass transfer Static mixer Aqueous two-phase system;

D O I：

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学科分类号：

摘要：

Recent technical advances in aqueous two-phase systems (ATPS) have made this a sound technique for the extraction of biomacromolecules. The extraction of α-amylase was investigated using aqueous two-phase systems formed by sodium sulphate–polyethylene glycol (PEG) in water in a 47-mm inner diameter spray column packed with three types of static mixers. The effects of dispersed-phase flow rate, phase composition, column height and diameter were studied. The extraction column was operated in a semi-batch manner. It was found that the hold-up and volumetric mass transfer coefficients increased with an increase in dispersed (PEG-rich) phase velocity and decreased with increasing phase composition. Empirical correlations were developed for fractional dispersed-phase hold-up and volumetric mass transfer coefficients.

引用

页码：169 / 178

页数：9