Molecular Analysis of the α-Galactosidase MEL Genes in Yeast Saccharomyces sensu stricto

被引：0
作者：
E. S. Naumova
I. V. Korshunova
G. I. Naumov
机构：
[1] State Research Center GosNIIgenetika,
来源：
Molecular Biology | 2003年 / 37卷
关键词：
sensu stricto; var. ; microevolution; α-galactosidases; sequencing; genes;
D O I：
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摘要：
To infer the molecular evolution of yeast Saccharomyces sensu stricto from analysis of the α-galactosidase MEL gene family, two new genes were cloned and sequenced from S. bayanus var. bayanus and S. pastorianus. Nucleotide sequence homology of the MEL genes of S. bayanus var. bayanus (MELb), S. pastorianus (MELpt), S. bayanus var. uvarum (MELu), and S. carlsbergensis (MELx) was rather high (94.1–99.3%), comparable with interspecific homology (94.8–100%) of S. cerevisiaeMEL1-MEL11. Homology of the MEL genes of sibling species S. cerevisiae (MEL1), S. bayanus (MELb), S. paradoxus (MELp), and S. mikatae(MELj) was 76.2–81.7%, suggesting certain species specificity. On this evidence, the α-galactosidase gene of hybrid yeast S. pastorianus (S. carlsbergensis) was assumed to originate from S. bayanus rather than from S. cerevisiae.
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页码：699 / 706
页数：7
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