Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting

被引：0
|
作者：
Ling Liu
Tom H Cheung
Gregory W Charville
Thomas A Rando
机构：
[1] Stanford University School of Medicine,The Glenn Center for the Biology of Aging and the Department of Neurology and Neurological Sciences
[2] Neurology Service and Rehabilitation,undefined
[3] Research and Development Center of Excellence,undefined
[4] Veterans Affairs (VA) Palo Alto Health Care System,undefined
[5] Present address: Division of Life Science,undefined
[6] Hong Kong University of Science and Technology,undefined
[7] Kowloon,undefined
[8] Hong Kong.,undefined
来源：
Nature Protocols | 2015年 / 10卷
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学科分类号：
摘要：
In this protocol, limb muscles are physically and enzymatically dissociated to maximally release resident mononucleated cells. Pure populations of either quiescent or activated muscle stem cells are then isolated by flow cytometry.
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页码：1612 / 1624
页数：12
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