MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland

被引：0
作者：
Olga Szaluś-Jordanow
Katarzyna Krysztopa-Grzybowska
Michał Czopowicz
Agata Moroz
Marcin Mickiewicz
Anna Lutyńska
Jarosław Kaba
Tomasz Nalbert
Tadeusz Frymus
机构：
[1] Warsaw University of Life Sciences-SGGW,Division of Infectious Diseases, Department of Small Animal Diseases with Clinic
[2] National Institute of Public Health-National Institute of Hygiene (NIPH-NIH),Department of Sera and Vaccine Evaluation
[3] Warsaw University of Life Sciences-SGGW,Laboratory of Veterinary Epidemiology and Economics
[4] The Cardinal Stefan Wyszyński Institute of Cardiology,Department of Medical Biology
来源：
Archives of Microbiology | 2018年 / 200卷
关键词：
subsp. ; MLST; RAPD; Goat; Morel’s disease;
D O I：
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学科分类号：
摘要：
Staphylococcus aureus subsp. anaerobius is an etiological agent of Morel’s disease in small ruminants. The infection results in superficial abscesses located near lymph nodes. In the study, molecular analysis based on multilocus sequence typing (MLST) of seven housekeeping genes (arcC, aroE, glp, gmk, pta, tpi, yqiL) and random amplified polymorphic DNA (RAPD) was carried out on 19 S. aureus subsp. anaerobius strains isolated from two different goat herds from Poland. All of the 19 S. aureus subsp. anaerobius strains were found to belong to single MLST and RAPD types which support the high clonality level of this agent. However, the results obtained show clearly that the S. aureus subsp. anaerobius clone found in goats in Poland is different from those previously described. However, it is identical to the ATCC 38844 strain isolated from sheep in Spain, which has not been so far genotyped using MLST.
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页码：1407 / 1410
页数：3
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共 84 条
[1]  
Alhendi AB(1993)An outbreak of abscess disease in goats in Saudi Arabia Zentralbl Veterinarmed A 40 646-651
[2]  
al-Sanhousi SM(1985) subsp. Int J Syst Bacteriol 35 99-102
[3]  
al-Ghasnawi YA(2011) subsp. nov., the causal agent of abscess disease of sheep Vet Microbiol 150 198-202
[4]  
Madawi M(1989) subsp. Rev Elev Med Vet Pays Trop 42 379-382
[5]  
De la Fuente R(2013) isolates from different countries are clonal in nature Stand Genomic Sci 9 1-13
[6]  
Suarez G(2000)Abscess disease in goats in the Sudan J Clin Microbiol 38 1008-1015
[7]  
Schleifer KH(1999) subsp. Acta Microbiol Pol 48 243-259
[8]  
De la Fuente R(2004) strain ST1464 genome sequence Vet Stanica Znan-Stručni Vet Časopis 35 139-144
[9]  
Ballesteros C(2000)Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of J Vet Med B Infect Dis Vet Public Health 47 55-62
[10]  
Bautista V(2010)Technical aspects of random amplified polymorphic DNA (RAPD) technique in genotyping of bacterial strains Folia Microbiol (Praha) 55 211-214
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