A Simplified Filter Paper Assay Method of Cellulase Enzymes Based on HPLC Analysis

被引：0
作者：
Deqiang Chu
Hongbo Deng
Xiaoxi Zhang
Jian Zhang
Jie Bao
机构：
[1] East China University of Science and Technology,State Key Laboratory of Bioreactor Engineering
[2] Jilin Economic Development Zone,Jilin Fuel Alcohol Co., PetroChina Corporation
来源：
Applied Biochemistry and Biotechnology | 2012年 / 167卷
关键词：
Filter paper activity; Cellulase; Simplified method; HPLC measurement; Corn stover;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A simplified filter paper assay (FPA) method of cellulase enzymes was proposed based on high-performance liquid chromatography (HPLC) measurement. The method was according to the sum of glucose and cellobiose concentrations measured by HPLC that was able to be correlated with filter paper units (FPU) of the cellulase enzymes assayed by the traditional FPA method, regardless of the differences in the sources, activities, and components of the cellulases. This simple and quick assay method for the cellulase enzymes provided another parameter of the ratio of glucose to cellobiose (G/C ratio) representing the capacity of cellulase enzymes degrading cellulose into fermentable monomeric sugars.
引用
收藏
页码：190 / 196
页数：6
相关论文
共 50 条
[1]  
Eveleigh ED(2009)undefined Biotechnology for Biofuels 2 21-268
[2]  
Mandels M(1987)undefined Pure and Applied Chimstry 59 257-749
[3]  
Andreotti R(2003)undefined Biotechnology and Bioengineering 82 745-1750
[4]  
Roche C(2010)undefined Applied Biochemstry and Biotechnology 162 1737-703
[5]  
Ghose TK(2003)undefined Applied Biochemistry and Biotechnology 105 789-1044
[6]  
Coward-Kelly G(2009)undefined Biotechnology and Bioengineering 102 1033-837
[7]  
Aiello-Mazzari C(2004)undefined Biotechnology and Bioengineering 88 832-175
[8]  
Kim S(1984)undefined Journal Chemical Technology and Biotechnology 34B 171-802
[9]  
Granda C(1984)undefined Biotechnology and Bioengineering 26 800-4488
[10]  
Holtzapple M(2011)undefined Bioresource Technology 102 4480-498
12345