Demonstration of testicular apoptosis in human male infertility states using a DNA laddering technique

被引:22
作者
Lin W.W. [1 ]
Lamb D.J. [1 ,2 ]
Lipshultz L.L. [1 ]
Kim E.D. [1 ]
机构
[1] Scott Department of Urology, Baylor College of Medicine
[2] Department of Molecular Biology, Baylor College of Medicine, Houston, TX
关键词
Public Health; Infertility; Morphometric Analysis; Infertility State; Biopsy Tissue;
D O I
10.1023/A:1007130320700
中图分类号
学科分类号
摘要
DNA laddering, morphometric analysis, and in-situ end labelling of testis biopsy tissue obtained from azoospermic or severely oligozoospermic men revealed increased apoptosis frequency, implicating a possible role of apoptosis in the pathogenesis of human male infertility.
引用
收藏
页码:361 / 370
页数:9
相关论文
共 22 条
[1]  
Allan, D.J., Harmon, B.V., Roberts, S.A., Spermatogonial apoptosis has three morphologically recognizable phases and shows no circadian rhythm during normal spermatogenesis in the rat (1992) Cell. Prolif., 25, p. 241
[2]  
Ansari, B., In-situ end labeling detects DNA strand breaks in apoptosis and other physiological and pathological states (1993) J. Pathol., 170
[3]  
Billing, H., Apoptosis in testis germ cells: Developmental changes in gonadotropin dependence and localization to selective tubule stages (1995) Endocrinology, 136 (1), p. 5
[4]  
Blanco-Rodriguez, J., Martinez-Garcia, C., Spontaneous germ cell death in the testis of the adult rat takes the form of apoptosis: Re-evaluation of the cell types that exhibit the ability to die during spermatogenesis (1996) Cell. Prolif., 29, p. 13
[5]  
Blendy, J.A., Severe impairment of spermatogenesis in mice lacking the CREM gene (1996) Mature, 380, p. 162
[6]  
Bursch, W., Oberhammer, F., Schulte-Hermann, R., Cell death by apoptosis and its protective role against disease (1992) Trends in Pharmacological Sciences, 13 (6), p. 245
[7]  
Gavrieli, Y., Sherman, Y., Ben-Sasson, S.A., Identification of programmed eell death in situ via specific labeling of nuclear DNA fragmentation (1992) J. Cell. Biol, 119, p. 493
[8]  
Henriksen, K., Hakovirta, H., Parvinen, M., Testosterone inhibits and induces apoptosis in rat seminiferous tubules in a stage-specific manner: In situ quantification in squash preparations after administration of ethane dimethane sulfonate (1995) Endocrinology, 136 (8), p. 3285
[9]  
Hikim, A.P.S., Wang, C., Leung, A., Swerdloff, R.S., Involvement of apoptosis in the induction of germ cell degeneration in adult rats after gonadotropin-releasing hormone antagonist treatment (1995) Endocrinology, 136 (6), p. 2770
[10]  
Huckins, C., The morphology and kinetics of spermatogonial degeneration in normal adult rats: An analysis using a simplified classification of the germinal epithelium (1978) Anat. Rec., 190, p. 905