Deficiency of Invariant NK T Cells in Crohn's Disease and Ulcerative Colitis

被引：0
作者：
Randall H. Grose
Fiona M. Thompson
Alan G. Baxter
Daniel G. Pellicci
Adrian G. Cummins
机构：
[1] University of Adelaide,Basil Hetzel Institute for Medical Research and Department of Medicine
[2] Adelaide,Comparative Genomics Centre
[3] and Department of Gastroenterology and Hepatology,Department of Pathology and Immunology
[4] James Cook University,Basil Hetzel Institute for Medical Research and Department of Medicine
[5] Monash University Central and Eastern Clinical School,undefined
[6] University of Adelaide,undefined
[7] Adelaide,undefined
[8] and Department of Gastroenterology and Hepatology,undefined
来源：
Digestive Diseases and Sciences | 2007年 / 52卷
关键词：
Crohn's disease; Invariant NK T cells; Ulcerative colitis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The aim of this study was to investigate whether immunoregulatory invariant NK T cells are deficient in Crohn's disease or ulcerative colitis. Blood was collected for flow cytometry from 106 Crohn's disease, 91 ulcerative colitis, and 155 control subjects. Invariant NK T cells were assessed by Vα24 and (α-galactosylceramide/CD1d tetramer markers. Intracellular cytokine was measured after in vitro anti-CD3 antibody stimulation. Vα24+ T cells were quantified in ileocolonic biopsies as mRNA by real-time PCR and by immunofluorescence. Circulating invariant NK T cells were 5.3% of the control levels in Crohn's (P < 0.001) and 7.9% of the control levels in ulcerative colitis (P < 0.001). Interleukin-4 production was impaired in Crohn's disease and ulcerative colitis. Intestinal Vα24 mRNA expression was 7% in Crohn's disease (P < 0.05) and 9% in ulcerative colitis (P < 0.05). Intestinal Vα24+ T cells were 23% in Crohn's disease but not reduced in ulcerative colitis. We conclude that invariant NK T cells are deficient in Crohn's disease and in ulcerative colitis.
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页码：1415 / 1422
页数：7
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