Molecular phylogenetic studies on Theileria spp. isolates (China) based on small subunit ribosomal RNA gene sequences

被引：0
作者：
Qin Liu
Yanqin Q. Zhou
Guosheng S. He
Marinda C. Oosthuizen
Danna N. Zhou
Junlong L. Zhao
机构：
[1] Huazhong Agricultural University,State Key Laboratory of Agricultural Microbiology
[2] Huazhong Agricultural University,College of Veterinary Medicine
[3] Shanghai Veterinary Research Institute,Department of Veterinary Tropical Diseases, Faculty of Veterinary Science
[4] University of Pretoria,National Institute of Parasitic Diseases
[5] Chinese Center for Disease Control and Prevention,undefined
来源：
Tropical Animal Health and Production | 2010年 / 42卷
关键词：
spp.; 18S rRNA; Phylogenetic tree;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Six Theileria spp. from cattle, buffalo and black goat were identified in the Hubei province of China. In order to study the taxonomic status of these parasites, phylogenetic analysis of 18S rRNA genes were carried out. The 18S rRNA genes from each isolate were amplified by the polymerase chain reaction and the approximate 1.75 kb products were cloned and sequenced. Phylogenetic analysis of these gene sequences revealed that the five parasites from buffalo and cattle belonged to the Theileria sergenti/buffeli/orientalis group. The parasite from the Chinese goat (Macheng-Hubei, DQ286802) was closely related to Theileria luwenshuni isolated from sheep in the north of China. This represent the first report on the use of molecular phylogeny to classify Theileria spp. obtained in the Hubei province, showing that Theileria spp. from ruminants found in Hubei province belongs to the benign group of Theileria spp.
引用
收藏
页码：109 / 114
页数：5
相关论文
共 127 条
[1]  
Ahmed JS(2002)Ticks and tick-borne diseases in Asia with special emphasis on China Parasitol. Res. 88 S51-S55
[2]  
Yin H(2006)Phylogenetic position of small-ruminant infecting piroplasms Ann. N. Y. Acad. Sci. 1081 498-504
[3]  
Schnittger L(2001)Bovine piroplasms in Minorca (Belearic Islands Spain): a comparison of PCR-based and light microscopy detection Vet. Parasitol. 99 249-259
[4]  
Jongejan F(1998)Nucleotide sequence heterogeneity in the small subunit ribosomal RNA gene variable (V4) region among and within geographic isolates of Theileria from cattle, elk and white-tailed deer Vet. Parasitol. 75 41-52
[5]  
Ahmed JS(1999)A study of the systematics of Parasitol. Res. 85 877-883
[6]  
Luo JX(1999) spp. based upon small-subunit ribosomal RNA gene sequences Vet. Parasitol. 83 99-105
[7]  
Schnittger L(2008)Molecular phylogenetic studies on Theileria parasites based on small subunit ribosomal RNA gene sequences Vet. Parasitol 151 327-331
[8]  
Seitzer U(2000)Molecular detection of Int. J. Parasitol. 30 943-952
[9]  
Jongejan F(2004) sp. in ticks and naturally infected sheep Vet. Parasitol. 120 339-345
[10]  
Yin H(2008)Molecular characterisation of the Parasitol. Res. 103 1407-1412
12345678910