Cellulose hydrolysis by immobilized Trichoderma reesei cellulase

被引:0
作者
Paetrice O. Jones
Palligarnai T. Vasudevan
机构
[1] University of New Hampshire,Department of Chemical Engineering, Kingsbury Hall
来源
Biotechnology Letters | 2010年 / 32卷
关键词
Bioethanol; Carrier-free immobilized cellulase; Cellulose; Ionic liquid;
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学科分类号
摘要
Cellulose hydrolysis by immobilized Trichoderma reesei cellulase in the presence of a low viscosity ionic liquid, 1-ethyl-3-methylimidazolium diethyl phosphate (EMIM-DEP), was investigated. Preparation of the carrier-free immobilized cellulase was optimized with respect to concentration of the cross-linker and the type of precipitant. The addition of 2% (v/v) EMIM-DEP during hydrolysis gave an initial reaction rate 2.7 times higher than the hydrolysis rate with no ionic liquid. The initial yield after 2 h was 0.7 g glucose/g cellulose, and the carrier-free immobilized cellulase (CFIC) was effectively re-used five times.
引用
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页码:103 / 106
页数:3
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