Cellulose hydrolysis by immobilized Trichoderma reesei cellulase

被引：0
作者：
Paetrice O. Jones
Palligarnai T. Vasudevan
机构：
[1] University of New Hampshire,Department of Chemical Engineering, Kingsbury Hall
来源：
Biotechnology Letters | 2010年 / 32卷
关键词：
Bioethanol; Carrier-free immobilized cellulase; Cellulose; Ionic liquid;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Cellulose hydrolysis by immobilized Trichoderma reesei cellulase in the presence of a low viscosity ionic liquid, 1-ethyl-3-methylimidazolium diethyl phosphate (EMIM-DEP), was investigated. Preparation of the carrier-free immobilized cellulase was optimized with respect to concentration of the cross-linker and the type of precipitant. The addition of 2% (v/v) EMIM-DEP during hydrolysis gave an initial reaction rate 2.7 times higher than the hydrolysis rate with no ionic liquid. The initial yield after 2 h was 0.7 g glucose/g cellulose, and the carrier-free immobilized cellulase (CFIC) was effectively re-used five times.
引用
收藏
页码：103 / 106
页数：3
相关论文
共 45 条
[1]  
Dadi AP(2006)Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step Biotechnol Bioeng 95 904-910
[2]  
Varanasi S(2006)Ethanol can contribute to energy and environmental goals Science 311 506-508
[3]  
Schall CA(2006)Ethanol’s energy return on investment: a survey of the literature 1990-present Environ Sci Technol 40 1744-1750
[4]  
Farrell AE(2008)Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media Biotechnol Lett 30 1037-1040
[5]  
Plevin RJ(2009)Improving enzymatic hydrolysis of wheat straw using ionic liquid 1-ethyl-3-methyl imidazolium diethyl phosphate pretreatment Bioresour Technol 100 3570-3575
[6]  
Turner BT(1991)Fuel ethanol from cellulosic biomass Science 251 1318-1323
[7]  
Jones AD(2005)Ethanol production using corn, switchgrass, and wood; biodiesel production using soybean and sunflower Nat Resour Res 14 65-76
[8]  
O’Hare M(2007)Cross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts Biochem Soc T 35 1583-1587
[9]  
Kammen DM(2003)Ionic liquid salt-induced inactivation and unfolding of cellulase from Green Chem 5 443-447
[10]  
Hammerschlag R(2007)Glucose solvation by the ionic liquid 1, 3-dimethylimidazolium chloride: a simulation study J Phys Chem B 111 13765-13774
12345