Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities

被引：0
作者：
Andrew Wilde
Sofia B. Lizarraga
Lijun Zhang
Christiane Wiese
Neal R. Gliksman
Claire E. Walczak
Yixian Zheng
机构：
[1] Howard Hughes Medical Institute,Department of Embryology
[2] Carnegie Institution of Washington,Department of Medical Genetics and Microbiology
[3] Graduate program in Biology,undefined
[4] Johns Hopkins University ,undefined
[5] Universal Imaging Corporation,undefined
[6] Medical Sciences Program,undefined
[7] Indiana University,undefined
[8] University of Toronto,undefined
来源：
Nature Cell Biology | 2001年 / 3卷
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摘要：
The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran–GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightfold. In addition to changing microtubule dynamics, Ran–GTP also alters the balance of motor activities, partly as a result of an increase in the amount of motile Eg5, a plus-end-directed microtubule motor that is essential for spindle formation. Thus, Ran regulates multiple processes that are involved in spindle assembly.
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页码：221 / 227
页数：6
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