Protein standard absolute quantification (PSAQ) method for the measurement of cellular ubiquitin pools

被引：0

作者：

Kaiser S.E. ^{[1
]}

Riley B.E. ^{[1
]}

Shaler T.A. ^{[2
]}

Trevino R.S. ^{[1
]}

Becker C.H. ^{[2
]}

Schulman H. ^{[2
]}

Kopito R.R. ^{[1
]}

机构：

[1] Department of Biology, Stanford University, Stanford, CA

[2] Caprion Proteomics U.S., LLC, Menlo Park, CA

来源：

Nature Methods | 2011年 / 8卷 / 8期

关键词：

D O I：

10.1038/nmeth.1649

中图分类号：

学科分类号：

摘要：

The protein ubiquitin is an important post-translational modifier that regulates a wide variety of biological processes. In cells, ubiquitin is apportioned among distinct pools, which include a variety of free and conjugated species. Although maintenance of a dynamic and complex equilibrium among ubiquitin pools is crucial for cell survival, the tools necessary to quantify each cellular ubiquitin pool have been limited. We have developed a quantitative mass spectrometry approach to measure cellular concentrations of ubiquitin species using isotope-labeled protein standards and applied it to characterize ubiquitin pools in cells and tissues. Our method is convenient, adaptable and should be a valuable tool to facilitate our understanding of this important signaling molecule. © 2011 Nature America, Inc. All rights reserved.

引用

页码：691 / 696

页数：5

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