Molecular characterization and PCR-based replicon typing of multidrug resistant Shigella sonnei isolates from an outbreak in Thimphu, Bhutan

被引：14
作者：
Ruekit S. [1 ]
Wangchuk S. [2 ]
Dorji T. [2 ]
Tshering K.P. [3 ]
Pootong P. [1 ]
Nobthai P. [1 ]
Serichantalergs O. [1 ]
Poramathikul K. [1 ]
Bodhidatta L. [1 ]
Mason C.J. [1 ]
机构：
[1] Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok
[2] Public Health Laboratory, Department of Public Health, Ministry of Health, Thimphu
[3] Department of Pediatrics, Jigme Dorji Wangchuk National Referral Hospital, Kawa Jangsa, Thimphu
来源：
BMC Research Notes | / 7卷 / 1期
关键词：
Bhutan; Multidrug resistance; Shigella sonnei;
D O I：
10.1186/1756-0500-7-95
中图分类号：
学科分类号：
摘要：
Background: Shigella species are an important cause of diarrhea in developing countries. These bacteria normally acquire their antibiotic resistance via several different mobile genetic elements including plasmids, transposons, and integrons involving gene cassettes. During a diarrhea surveillance study in Thimphu, Bhutan in June and July, 2011, Shigella sonnei were isolated more frequently than expected. This study describes the antibiotic resistance of these S. sonnei isolates. Methods. A total of 29 S. sonnei isolates from Thimphu, Bhutan was characterized for antimicrobial susceptibility by disc diffusion assay and minimum inhibitory concentration (MIC) assay. All isolates were tested by pulsed-field gel electrophoresis (PFGE) with restriction enzyme XbaI and were tested for plasmid. The plasmid patterns and the PFGE patterns were analyzed by Bionumerics software. DNA sequencing was performed on amplified products for gyraseA gene and class 1 and class 2 integrons. S. sonnei isolates were classified for incompatibility of plasmids by PCR-based replicon typing (PBRT). Results: These S. sonnei were resistant to multiple drugs like ciprofloxacin, nalidixic acid, trimethoprim-sulfamethoxazole, streptomycin, and tetracycline but susceptible to azithromycin. All isolates had class 2 integrons dfrA1, sat1 and aadA1 genes. Two point mutations in Gyrase A subunit at position Ser83Leu and Asp87Gly were detected in these quinolone resistant isolates. The plasmid and PFGE patterns of S. sonnei isolates suggested a clonal relationship of the isolates. All isolates carried common ColE plasmid. ColE plasmid co-resided with B/O plasmid (nine isolates) or I1 plasmid (one isolate). Conclusions: The characteristics of 29 S. sonnei isolates from Thimphu, Bhutan in June and July, 2011 are identical in PFGE, plasmid and resistance pattern. This study suggests that these recent S. sonnei isolates are clonally related and multidrug-resistant. © 2014 Ruekit et al.; licensee BioMed Central Ltd.
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共 32 条
[1]  
Fact Sheets: Diarrhoeal Disease 2013
[2]  
Dzongkha Population Projections 2006-2015: National Statistics Bureau
[3]  
Annual Health Bulletin 2009
[4]  
Annual Health Bulletin 2011
[5]  
Darnal J.B., Nepal H.K., Damchu, Wangchuk L.Z., Doung-Ngern P., Swaddiwudhipong W., An Outbreak of Shigellosis in a Remote Village of Mongar District, Bhutan from March to April 2011, OSIR, 5, 2, pp. 1-8, (2012)
[6]  
Rowe-Magnus D.A., Guerout A.M., Mazel D., Bacterial resistance evolution by recruitment of super-integron gene cassettes, Mol Microbiol, 43, 6, pp. 1657-1669, (2002)
[7]  
Gravel A., Fournier B., Roy P.H., DNA complexes obtained with the integron integrase IntI1 at the attI1 site, Nucleic Acids Res, 26, 19, pp. 4347-4355, (1998)
[8]  
White P.A., McIver C.J., Rawlinson W.D., Integrons and gene cassettes in the enterobacteriaceae, Antimicrob Agents Chemother, 45, 9, pp. 2658-2661, (2001)
[9]  
Delappe N., O'Halloran F., Fanning S., Corbett-Feeney G., Cheasty T., Cormican M., Antimicrobial resistance and genetic diversity of Shigella sonnei isolates from western Ireland, an area of low incidence of infection, J Clin Microbiol, 41, 5, pp. 1919-1924, (2003)
[10]  
Denap J.C., Hergenrother P.J., Bacterial death comes full circle: Targeting plasmid replication in drug-resistant bacteria, Org Biomol Chem, 3, 6, pp. 959-966, (2005)
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