The contribution of gut bacteria to insecticide resistance and the life histories of the major malaria vector Anopheles arabiensis (Diptera: Culicidae)

被引:0
|
作者
Kirsten Barnard
Alexander C. S. N. Jeanrenaud
Basil D. Brooke
Shüné V. Oliver
机构
[1] University of the Witwatersrand,School of Molecular and Cell Biology, Faculty of Science
[2] National Institute for Communicable Diseases of the National Health Laboratory Service,Centre for Emerging Zoonotic and Parasitic Diseases
[3] University of the Witwatersrand,Wits Research Institute for Malaria, MRC Collaborating Centre for Multi
来源
Scientific Reports | / 9卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
The gut microbiota of mosquitoes is a crucial determinant of their fitness. As such, the biology of the gut microbiota of Anopheles arabiensis, a major malaria vector of Southern Africa, was investigated. Two laboratory strains of An. arabiensis were used; SENN, an insecticide susceptible strain, and SENN-DDT, a resistant strain. The strains were supplemented with either non-commensal bacteria or antibiotics via a sucrose source to sterilize the gut. The strains were fed the broad-spectrum bactericidal antibiotic gentamicin, or a preferentially gram-positive bactericidal (vancomycin), gram-negative bactericidal (streptomycin) or broad-spectrum bacteriostatic (erythromycin), either by sugar supplementation or by artificially-spiked blood-meal. The effects on adult mosquito longevity and insecticide resistance phenotype were assessed. Bacteria from the midgut of both strains were characterised by MALDI-TOF mass spectroscopy. Bactericidal antibiotics increased longevity in SENN-DDT. Bacterial supplementation increased insecticide tolerance. Antibiotic supplementation via sugar decreased tolerance to the insecticides deltamethrin and malathion. Blood-supplemented vancomycin decreased insecticide resistance, while gentamicin and streptomycin increased resistance. SENN showed a greater gut bacterial diversity than SENN-DDT, with both strains dominated by Gram-negative bacteria. This study suggests a crucial role for bacteria in An. arabiensis life history, and that gut microflora play variable roles in insecticide resistant and susceptible mosquitoes.
引用
收藏
相关论文
共 50 条
  • [21] The Current Insecticide Resistance in Main Malaria Vector Anopheles arabiensis in Yemen
    Al-Koleeby, Zalalham
    El Aboudi, Ahmed
    Assada, Mithaq
    Al-Hadi, Mohamed
    Abdalr Ahman, Mohammed
    Awash, Abdullah
    Ahmed, Abdul Samad
    Mohamedi, Hani
    Al Jarbany, Jamil
    Faraj, Chafika
    JOURNAL OF TROPICAL MEDICINE, 2020, 2020
  • [22] Effects of inorganic fertilizer on larval development, adult longevity and insecticide susceptibility in the malaria vector Anopheles arabiensis (Diptera: Culicidae)
    Samuel, Michael
    Brooke, Basil D.
    Oliver, Shune V.
    PEST MANAGEMENT SCIENCE, 2020, 76 (04) : 1560 - 1568
  • [23] Stable chromosomal inversion Polymorphisms and insecticide resistance in the malaria vector mosquito Anopheles gambiae (Diptera: Culicidae)
    Brooke, BD
    Hunt, RH
    Chandre, F
    Carnevale, P
    Coetzee, M
    JOURNAL OF MEDICAL ENTOMOLOGY, 2002, 39 (04) : 568 - 573
  • [24] Insecticide resistance in the malaria vector Anopheles arabiensis in Mamfene, KwaZulu-Natal
    Brooke, Basil D.
    Robertson, Leanne
    Kaiser, Maria L.
    Raswiswi, Eric
    Munhenga, Givemore
    Venter, Nelius
    Wood, Oliver R.
    Koekemoer, Lizette L.
    SOUTH AFRICAN JOURNAL OF SCIENCE, 2015, 111 (11-12) : 190 - 192
  • [25] Zooprophylaxis as a control strategy for malaria caused by the vector Anopheles arabiensis (Diptera: Culicidae): a systematic review
    Abebe Asale
    Luc Duchateau
    Brecht Devleesschauwer
    Gerdien Huisman
    Delenasaw Yewhalaw
    Infectious Diseases of Poverty, 6
  • [26] Pyrethroid resistance in the major malaria vector Anopheles arabiensis in Nouakchott, Mauritania
    Aichetou Mint Mohamed Lemine
    Mohamed Aly Ould Lemrabott
    El Hadji Amadou Niang
    Leonardo K. Basco
    Hervé Bogreau
    Ousmane Faye
    Ali Ould Mohamed Salem Boukhary
    Parasites & Vectors, 11
  • [27] Pyrethroid resistance in the major malaria vector Anopheles arabiensis in Nouakchott, Mauritania
    Lemine, Aichetou Mint Mohamed
    Lemrabott, Mohamed Aly Ould
    Niang, El Hadji Amadou
    Basco, Leonardo K.
    Bogreau, Herve
    Faye, Ousmane
    Boukhary, Ali Ould Mohamed Salem
    PARASITES & VECTORS, 2018, 11
  • [28] Zooprophylaxis as a control strategy for malaria caused by the vector Anopheles arabiensis (Diptera: Culicidae): a systematic review
    Asale, Abebe
    Duchateau, Luc
    Devleesschauwer, Brecht
    Huisman, Gerdien
    Yewhalaw, Delenasaw
    INFECTIOUS DISEASES OF POVERTY, 2017, 6
  • [29] ECOLOGY OF THE MALARIA VECTOR ANOPHELES-ARABIENSIS PATTON (DIPTERA, CULICIDAE) BY THE NILE IN NORTHERN SUDAN
    DUKEEN, MYH
    OMER, SM
    BULLETIN OF ENTOMOLOGICAL RESEARCH, 1986, 76 (03) : 451 - 467
  • [30] THE WEST AFRICAN KNOCK DOWN RESISTANCE MUTATION DETECTED IN THE PRIMARY MALARIA VECTOR ANOPHELES ARABIENSIS (DIPTERA: CULICIDAE) IN ETHIOPIA
    Woldesilasse, Esayas Kinfe
    Tekie, Habte T.
    Seth, Irish R.
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2019, 101 : 441 - 442