Increased Yield of Residual γH2AX Foci in p53-Deficient Human Lung Carcinoma Cells Exposed to Subpicosecond Beams of Accelerated Electrons

被引：0
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作者：
N. Yu. Vorobyeva
N. S. Babayan
B. A. Grigoryan
A. A. Sargsyan
L. G. Khondkaryan
L. S. Apresyan
A. K. Chigasova
E. I. Yashkina
D. V. Guryev
S. M. Rodneva
A. A. Tsishnatti
Yu. A. Fedotov
R. M. Arutyunyan
A. N. Osipov
机构：
[1] Russian Academy of Sciences,N. N. Semenov Federal Research Center of Chemical Physics
[2] Federal Medical-Biological Agency of Russia,A. I. Burnasyan Federal Medical Biophysical Center
[3] Institute of Molecular Biology,N. M. Emanuel Institute of Biochemical Physics
[4] National Academy of Science of the Republic of Armenia,undefined
[5] Yerevan State University,undefined
[6] CANDLE Synchrotron Research Institute,undefined
[7] Russian Academy of Sciences,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2022年 / 172卷
关键词：
subpicosecond electron irradiation; DNA double strand breaks; residual γH2AX foci; p53; human lung carcinoma;
D O I：
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摘要：
We studied quantitative yield of residual (24 h post-irradiation) phosphorylated histone (γH2AX) foci as a marker of DNA double strand breaks in wild-type A549 and p53-deficient H1299 human lung carcinoma cells after exposure to subpicosecond (energy 4 MeV, pulse duration 400 fsec, peak dose rate during the pulse 16 GGy/s) and quasi-continuous (energy 3.6 MeV) beams of accelerated electrons in a dose range of 0.5-10.0 Gy. The efficiency of pulse irradiation in A549 and H1299 cells assessed by the yield of residual foci was higher than the efficiency of quasi-continuous exposure by 1.8 and 5.3 times, respectively. Significant differences in quantitative yield of residual γH2AX foci between wild-type and p53-deficient cell lines were observed only after exposure to subpicosecond, but not quasi-continuous beams of accelerated electrons.
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页码：756 / 759
页数：3
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