An improved protocol for bacteria identification by MALDI-TOF MS directly from positive blood cultures

被引：0
作者：
Sara Cruz
David Abreu
Rosário Gomes
Inês Martins-Oliveira
Ana Silva-Dias
Blanca Perez-Viso
Rafael Cantón
Cidália Pina-Vaz
机构：
[1] University of Porto,Division of Microbiology, Department of Pathology, Faculty of Medicine
[2] FASTinov SA,CINTESIS
[3] Porto,Center for Health Technology and Services Research, Faculty of Medicine
[4] University of Porto,Servicio de Microbiología
[5] Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS),CIBER de Enfermedades Infecciosas (CIBERINFEC)
[6] Instituto de Salud Carlos III,undefined
来源：
European Journal of Clinical Microbiology & Infectious Diseases | 2024年 / 43卷
关键词：
MALDI-TOF; Mass spectrometry; Blood cultures; Rapid identification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
FASTinov® developed a rapid antimicrobial susceptibility test that includes the purification of a bacterial suspension directly from positive blood cultures (BC). In order to streamline laboratory workflow, the use of the bacterial suspension obtained through FASTinov® sample prep was tested for identification (ID) by matrix absorption laser deionization–time of flight mass spectrometry (MALDI-TOF MS) (Bruker) in 364 positive BC, and its accuracy assessed comparing with the MALDI-TOF MS ID of the next-day subcultured colonies. FASTinov sample prep was highly reliable for rapid ID directly from BC with proportion of agreement of 94.9% for Gram-positive and 96.3% for Gram-negative bacteria.
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页码：605 / 610
页数：5
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