Full-size form of human liver AMP-deaminase?

被引：0
作者：
M. Szydlowska
Z. Chodorowski
I. Rybakowska
G. Nagel-Starczynowska
K. Kaletha
机构：
[1] Medical University of Gdansk,Department of Biochemistry
[2] Medical University of Gdansk,I Clinic of Internal Medicine and Toxicology
[3] Medical University of Gdansk,Department of Pharmaceutical Biochemistry
来源：
Molecular and Cellular Biochemistry | 2004年 / 266卷
关键词：
AMP-deaminase; human liver;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
AMP-deaminase from human liver was purified by two-step phosphocellulose chromatography, and SDS-PAG electrophoresis of the most active enzyme fraction eluted has been performed. The largest of the protein fragments revealed had a size (92 kDa) of an apparent full-size enzyme subunit, and reacted positively with antibodies produced against specific human ampd2 gene product. Three-day storage at cold room temperature modified significantly the electrophoretical pattern of the enzyme, evidencing continuous and progressive degradation of its structure. This is a first report evidencing the presence of apparent full-size form of human liver AMP-deaminase in preparation obtained from endogenous source (Mol Cell Biochem 266: 133–137, 2004)
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页码：133 / 137
页数：4
相关论文
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