Full-size form of human liver AMP-deaminase?

被引：0

作者：

M. Szydlowska

Z. Chodorowski

I. Rybakowska

G. Nagel-Starczynowska

K. Kaletha

机构：

[1] Medical University of Gdansk,Department of Biochemistry

[2] Medical University of Gdansk,I Clinic of Internal Medicine and Toxicology

[3] Medical University of Gdansk,Department of Pharmaceutical Biochemistry

来源：

Molecular and Cellular Biochemistry | 2004年 / 266卷

关键词：

AMP-deaminase; human liver;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

AMP-deaminase from human liver was purified by two-step phosphocellulose chromatography, and SDS-PAG electrophoresis of the most active enzyme fraction eluted has been performed. The largest of the protein fragments revealed had a size (92 kDa) of an apparent full-size enzyme subunit, and reacted positively with antibodies produced against specific human ampd2 gene product. Three-day storage at cold room temperature modified significantly the electrophoretical pattern of the enzyme, evidencing continuous and progressive degradation of its structure. This is a first report evidencing the presence of apparent full-size form of human liver AMP-deaminase in preparation obtained from endogenous source (Mol Cell Biochem 266: 133–137, 2004)

引用

页码：133 / 137

页数：4

共 65 条

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