Identifying and selecting for genetic diversity in Papua New Guinea sweetpotato Ipomoea batatas (L.) Lam. germplasm collected as botanical seed

被引：12

作者：

Fajardo D.S. ^{[1
]}

La Bonte D.R. ^{[1
]}

Jarret R.L. ^{[2
]}

机构：

[1] Department of Horticulture, LA State Univ. Agricultural Center, LA Agricultural Experiment Station, Baton Rouge

[2] United States Dept. of Agriculture, Agricultural Research Service, Griffin

来源：

Genetic Resources and Crop Evolution | 2002年 / 49卷 / 5期

关键词：

AFLP; Ipomoea batatas; Maximally diverse subset; Molecular marker;

D O I：

10.1023/A:1020955911675

中图分类号：

学科分类号：

摘要：

A total of 141 Ipomoea batatas (L.) Lam. accessions derived from botanical seed originally collected from 26 sites in 4 Provinces in Papua New Guinea, a secondary center of genetic diversity for sweetpotato, were genetically analyzed. Two hundred Amplified Fragment Length Polymorphism (AFLP) markers were identified and utilized in the analysis. Relatedness among accessions was estimated by analyzing the AFLP data using the Dice coefficient of similarity and UPGMA methods. The molecular analysis revealed relatively limited genetic diversity within and between sites. Genotypes collected in a given region often displayed molecular marker variability similar to that observed over the entire sampled area. However, a subset of 14 genotypes derived from seed collected from New Ireland island differed from genotypes collected on New Guinea island. Estimates of genetic diversity-based similarity values calculated from the AFLP data indicated a moderate level of diversity (0.767 mean coefficient of similarity) across all plant materials analyzed. Three methods of selection were evaluated for their efficacy in capturing the molecular marker diversity within the plant materials in the form of a subset. They were random, stratified-random (geographic based), and marker-assisted selection (MAS). MAS was the most efficient. A Maximally Diverse Subset (MDS) of 12 genotypes capturing 92% of the molecular marker diversity was identified.

引用

页码：463 / 470

页数：7

共 25 条

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