High-Speed Counter-Current Chromatography Combined with Column Chromatography for Isolation of Methyllycaconitine from Delphinium pseudocyanthum

被引：0
作者：
DongYu Gu
Yi Yang
Jie Zhong
Haji Akber Aisa
TianYou Zhang
机构：
[1] Xinjiang Technical Institute of Physics and Chemistry,Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry
[2] Chinese Academy of Sciences,undefined
[3] Graduate University of the Chinese Academy of Sciences,undefined
[4] Beijing Institute of New Technology Application,undefined
来源：
Chromatographia | 2007年 / 66卷
关键词：
High-speed counter-current chromatography; Methyllycaconitine;
D O I：
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中图分类号：
学科分类号：
摘要：
Preparative high-speed counter-current chromatography (HSCCC) combined with conventional column chromatography (CC) has been used for isolation and purification of methyllycaconitine from Delphinium pseudocyanthum. n-Hexane-ethyl acetate-methanol-water, 1:1:1:2 (v/v), was used as the solvent system for HSCCC. Separation of methyllycaconitine from an HSCCC fraction was successfully achieved by CC on silica gel using chloroform-methanol, 7:1 (v/v), as mobile phase. A total of 113.45 mg methyllycaconitine of purity >95% was obtained from 1.044 g extract of D. pseudocyanthum. Its structure was identified by MS and NMR.
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页码：949 / 951
页数：2
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