Effects of tris(2,3-dibromopropyl) isocyanurate on steroidogenesis in H295R cells

被引：0

作者：

Xun Li

Yu Pan

Chang Wang

Minjie Chen

Yuchen Liu

Jia Li

Zhen Zhou

Jinhua Xu

Yong Liang

Maoyong Song

机构：

[1] Jianghan University,School of Medicine

[2] Jianghan University,Institute of Environment and Health

[3] Jianghan University,Key Laboratory of Optoelectronic Chemical Materials and Devices, Ministry of Education, School of Chemical and Environmental Engineering

[4] Chinese Academy of Sciences,State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco

来源：

Environmental Earth Sciences | 2016年 / 75卷

关键词：

Tris(2,3-dibromopropyl) isocyanurate; H295R; Sex hormone biosynthesis; Steroidogenic gene; Endocrine-disrupting action;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Tris(2,3-dibromopropyl) isocyanurate (TBC) is widely used in polymer products. It is ubiquitously found in environmental matrices. Previous studies have shown that TBC has potential endocrine-disrupting effects on aquatic organisms. However, the effects on adrenocortical function and the underlying mechanisms are not well characterized. In present study, the H295R cell line was employed to investigate the potential endocrine-disrupting action of TBC. TBC inhibited basal and forskolin-induced sex hormone production but did not exhibit cytotoxicity. The expression of the steroidogenic genes, StAR, 3βHSD2, CYP17, CYP21, CYP19, HMGR, 17βHSD1, 17βHSD4 and CYP11A, was not changed upon TBC exposure. However, the cAMP inducer forskolin strongly induced the expression of all these genes, except for HMGR, 17βHSD1 and 17βHSD4. TBC blocked forskolin-induced StAR, 3βHSD2, CYP17, CYP21 and CYP19 mRNA expression. Our results indicate that TBC can inhibit sex hormone biosynthesis due to the interference with steroidogenic gene transcription in activated condition.

引用

共 294 条

[1]

Birnbaum LS(2004)Brominated flame retardants: cause for concern? Environ Health Perspect 112 9-455

[2]

Staskal DF(2005)Inhibition and induction of aromatase (CYP19) activity by brominated flame retardants in H295R human adrenocortical carcinoma cells Toxicol Sci 88 447-550

[3]

Cantón RF(1996)Forskolin treatment directs steroid production towards the androgen pathway in the NCI-H295R adrenocortical tumour cell line Endocr Res 22 545-1067

[4]

Sanderson JT(2007)Developmental neurotoxicity of polybrominated diphenyl ether (PBDE) flame retardants NeuroToxicology. 28 1047-233

[5]

Letcher RJ(2006)Levels and trends of brominated flame retardants in the Arctic Chemosphere 64 209-772

[6]

Bergman Å(2007)Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line Environ Toxicol Chem 26 764-120

[7]

van den Berg M(2008)Nitrophenols isolated from diesel exhaust particles regulate steroidogenic gene expression and steroid synthesis in the human H295R adrenocortical cell line Toxicol Appl Pharmacol 229 109-5496

[8]

Cobb VJ(1990)Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis Cancer Res 50 5488-305

[9]

Williams BC(2006)The H295R system for evaluation of endocrine-disrupting effects Ecotoxicol Environ Saf 65 293-164

[10]

Mason JI(2015)Disruption of iron homeostasis and resultant health effects upon exposure to various environmental pollutants: a critical review J Environ Sci 34 155-186

← 1 2 3 4 5 6 7 8 9 10 →