The First Nucleotide of the Codon Located in the P Site of the 80S Ribosome Is Close to G1702 of the 18S rRNA as Revealed by Crosslinking to a pUUUGUU Derivative Containing a Perfluorophenylazido Group at the Guanine

被引：0
作者：
N. A. Demeshkina
M. N. Repkova
A. G. Ven'yaminova
D. M. Graifer
G. G. Karpova
机构：
[1] Institute of Chemical Biology and Fundamental Medicine,Siberian Division, Russian Academy of Sciences
来源：
Molecular Biology | 2004年 / 38卷
关键词：
80S ribosome; affinity modification; mRNA analog; A and P ribosome sites; 18S rRNA;
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摘要：
Modification of the 18S rRNA with a pUUUGUU derivative carrying a perfluorophenylazido group at N7 of G was studied in the complex with the human 80S ribosome and Val-tRNAVal, which directs modified GUU to the P site. Reverse transcription reported modification of invariant G1702 of the 18S rRNA. On evidence of the results and the earlier data on affinity modification of the human ribosome with tetra- or heptaribonucleotide derivatives carrying an alkylating group at the 3′ end, the template was assumed to make a bend between the A- and P-site codons, which brings both codons closer to G1702 of the 18S rRNA.
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页码：421 / 426
页数：5
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